Experimental Research On MtDNA And Respiratory Chain Complex Kinase In Heart And Brain Of Rats With Spleen Qi Deficiency, Spleen Yang Deficiency And Spleen Yin Deficiency Syndrome And Effective Mechanism Of Chinese Herbs For Reinforcing Spleen

In company with advancement of society, per capita lifetime become more and more long, tendency of aging has become universal phenomenon of present world, it is undoubtedly the most urgent medicinal problem to prevent premature senility, postpone decrepitude, and prolong lifetime and increase quality of life. Traditional Chinese Medicine has abundant clinical experience and form theoretical system to postpone decrepitude, it emphasize that spleen is the root of after-birth and the source of qi and blood. Clinical practice also show that once human body is in the state of spleen deficiency, production of qi and blood would be deficiency, function of each internal organs would be weakened, metabolism would be depressed or maladjustment, then a series of pathology change resemble with decrepitude would appear.It has also been proved by clinical experience that growth and development of human body depend on nourishing of after-birth, the degree of decrepitude is also influenced by nourishing of after-birth. So the growth and development of human body has important relationship with spleen, spleen deficiency is the basis of subsidence of all function and appearance of decrepitude to human body. Therefore, researching the relationship between spleen deficiency and decrepitude and investigating its effective mechanism have most important realistic significance in the course of medical practice to postpone decrepitude.Development of realistic science has proved that researching microcosmic mechanism under macroscopical phenomenon is the important principle to impulse and cause advancement of science finally. So contemporary mechanism research on TCM syndrome or symptom complex is the true way to develop TCM.Modern medicine consider that the main character of decrepitude is descend of systemic physiological function along with increase of age, in the same time mitochondria is not only the energy basis of cell activity but also the energybasis of physiological function of all the body, it has important function in the course of decrepitude, apoptosis and sequencing death, so decline of mitochondria is the foundation of the course of decrepitude.It has been proved that tissue and organ whose metabolism is bloom such as cardiovascular system and central nervous system etc. are the place to appear decrepitude firstly, it because that mtDNA in these place is more and easy to be damaged. Nowadays, in many disease and the course of decrepitude, mutation of mtDNA has been found, and this change of mtDNA formation can cause decline of metabolism function, the latter not only appears reduce of ATP compound which cause physiological function decline of cell and all the body but also appears gathering of oxidizer such as free radical which cause the decline of the former and tissue in all the body. Thereby, Linmane (American) advance "Decrepit Mitochondria Theory" .The relationship of spleen deficiency and decrepitude is osculating, and the relationship of mitochondria and decrepitude is more and more definitude too. The research of relationship between spleen deficiency and structure and function of mitochondria is helpful to reveal the internal contact between spleen deficiency and decrepitude, then reveal molecular biology foundation of "spleen is the basis of after-birth" through pathology change of spleen deficiency, open up new method to postpone decrepitude by Chinese herbs.Based on previous research, this experiment is mainly research the deficiency of mtDNA in heart and brain of old rats with spleen deficiency and the activity of respiratory chain complex kinase. Combine with previous research result, discuss the change disciplinarian of mtDNA deficiency and respiratory chain complex kinase activity, and the molecular biology mechanism of postpone decrepitude by Chinese herbs for reinforcing spleen. The main content of this research includes:1. Observe the change of mtDNA deficiency in heart and brain of old rats with spleen qi deficiency, spleen yang deficiency and spleen yin deficiency, and the intervening fun...