Research On Key Technologies Of Multi-Phase Dual-Channel Bioprinting

In recent years,with the advancement of science and technology and the continuous improvement of medical level,there are new treatments for many major diseases.For tissue and organ necrosis or defect,organ transplantation technology is becoming more and more mature.However,insufficient organ donors for transplantation and immune rejection caused by allogeneic transplantation have become the main bottlenecks restricting organ transplantation technology.In order to solve this bottleneck,many researchers have tried to use bioprinting technology to use cells,growth factors,and biomaterials as printing materials,hoping to create bioactive biomimetic tissues or organs to replace transplanted tissues or organs.Although the research in this field has achieved fruitful results,there are still many problems to be solved in key factors such as bioprinting materials,preparation processes,and 3D printing equipment involved in bioprinting technology.Aiming at the multi-cell precision bioprinting technology,this topic proposes a high-precision multi-phase dual-channel bioprinting equipment solution for osteochondral.According to the requirements of multi-cell accurate printing,the hardware parts such as its three-dimensional mechanical structure,hardware control system,and functionalized nozzles of various materials are designed,and the characteristic frequency of the three-dimensional structure is calculated by the method of simulation analysis.Based on the bioprinting system,this study conducted printing tests on common osteochondral repair materials,and optimized the printing parameters.The results show that the printer has high precision,reliability and stability.The double-layer porous structure scaffold was prepared by the bioprinter developed in this subject,and the cell adhesion on the scaffold and the induction of bone differentiation were tested by cell experiments.The CCK8 experiment was performed on the samples to test the cell proliferation.The results show that the three-dimensional structure is conducive to the growth and proliferation of cells,thus verifying that the biological 3D printer developed in this subject has good functionality,and the research results provide strong theoretical and technical support for further technology research and development in this field.