Effects Of Qingjiezhichu Oral Solution On AC/cAMP/PKA Signaling Pathway In TD Model Rat

Objective: To investigate the effects of Qingjie Zhichou oral liquid on behavior and DRD1,DRD2,AC/cAMP/PKA signaling pathways in TD rats,and to preliminarily explore its mechanism.Methods: Forty SD rats were randomly divided into blank group(n=10)and model group(n=30).Ten rats in the blank group were intraperitoneally injected with normal saline.Thirty model rats were established by intraperitoneal injection of 7-day IDPN.After successful modeling,the rats were randomly divided into model group,western medicine group and Chinese medicine group,with 10 rats in each group,and then treated with drugs.The blank group and the model group were treated with sterilized distilled water,the western medicine group was treated with Haloperidol,and the Chinese medicine group was treated with Qingjie Zhichou oral liquid for 14 days.The body weight,stereotypical behavior score and motor behavior score of the rats were recorded at 7 days after modeling and 7、 14 days after intragastric administration.The content of DRD1、 DRD2、 AC、cAMP and PKA were detected in the striatum of rats after 14 days of experimental treatment.Results: After 14 days of gavage,the scores of motor behavior and stereotyped behavior in western medicine group and Chinese medicine group were significantly lower than those in model group(P<0.01).The contents of DRD1 、DRD2、AC、cAMP and PKA in the striatum of rats model group,western medicine group and Chinese medicine group were lower than those of rats blank group(P<0.01).The contents of DRD1 、 DRD2 、 AC 、cAMP and PKA in the striatum of rats western medicine group and Chinese medicine group were higher than those of rats model group(P<0.01).There were no significant differences in the contents of DRD1 、DRD2、AC、cAMP and PKA in the striatum of rats western medicine group and Chinese medicine group(P>0.05).Conclusion: Qingjie Zhichou oral liquid can obviously improve the motor behavior and rigid behavior of TD model rats,and it’s mechanism may be related to the regulation of dopamine metabolism pathway in cortico-striato-thalamo-cortical loop.