| Purpose:Briefly describe and record Professor Gao Tianshu’s academic thoughts and clinical medical records on clinical treatment of thyroid diseases,and use the method of in vivo experiments to detect and compare the gene and protein expression levels of thyroid hormone in serum and NIS,TPO in thyroid tissue,and verify and compare the effects of iodine rich traditional Chinese medicine commonly used by Professor Gao Tianshu and iodine rich traditional Chinese medicine and sodium iodide on iodine escape mechanism in rats.This provides new ideas and methods for better exploring the mechanism of iodine rich traditional Chinese medicine compound in treating thyroid diseases in the future.Material and method:64 experimental Wistar rats(6-8 weeks old)were fed adaptively for 2 weeks using distilled water for 1 and 6 days,iodine rich Chinese herbal compound for 1and 6 days,iodine rich Chinese herbal compound for 1 and 6 days,and sodium iodide for 1and 6 days.Each group of animals was gavaged once a day.Weigh after the last gastric gavage and euthanize on an empty stomach overnight.Take samples,separate animal serum,take a portion of the blood sample,use a separation tube to separate the serum and agglutinate for 30 minutes,centrifuge for 20 minutes at 1000 rpm/10 minutes,separate the serum,filter for sterilization at 0.22 um,and store T3,T4,and TSH to be tested at-80 ℃.Take rat thyroid tissue and divide it into 1.8ml cryopreservation tubes.Store it in liquid nitrogen at-80 ℃ for detection of NIS and TPO genes and protein expression in thyroid tissue using RT-PCR and Western blot protein blotting.Results:1.EIA measurement of serum T3 levels: Except for the sodium iodide group,the trend of T3 levels in other groups was not significant.The specific differences were as follows: the sodium iodide group had a statistically significant decrease in T3 levels compared to the control group after 1 day of medication,P<0.01;Compared with the control group,the T3 level in the iodine rich Chinese medicine group increased one day after application,with a statistically significant difference(P<0.05).After 6 days of administration of iodine rich traditional Chinese medicine compound,the T3 level decreased compared to 1 day of administration,with a statistically significant difference(P<0.01);The other two medication groups showed a statistically significant increase in T3 levels after 6 days of medication compared to 1 day of medication,with a P<0.01 difference.2.EIA measurement of serum T4 levels: After 1 day of medication,the T4 levels in each medication group decreased compared to the control group.However,after 6 days of medication,the T4 levels increased compared to 1 day of medication.The specific differences are as follows: After 1 day of medication,the T4 levels in each medication group were significantly reduced compared to the control group,and the difference was statistically significant,P<0.01;The T4 levels of iodine rich traditional Chinese medicine and sodium iodide increased significantly after 6 days of administration compared to 1 day of administration,with a statistical difference of P<0.01.3.EIA measurement of serum TSH level results: The trend of TSH level change in each group is not significant,and the specific differences are as follows: after 1 day of medication,the TSH level in the iodine rich traditional Chinese medicine group decreased compared to the control group,with a statistically significant difference(P<0.01);The TSH levels in the iodine rich traditional Chinese medicine compound group and sodium iodide group increased after 6days of administration compared to 1 day of administration,with a statistically significant difference(P<0.01).4.RT-PCR measurement of NIS m RNA results: Compared with the control group,the sodium iodide group showed a decrease in NIS m RNA levels after 1 day of medication,P<0.01;There was no significant statistical difference in NIS m RNA levels between the other two drug groups after 1 day of medication compared to the control group.After 6 days of medication,the levels of NIS m RNA in each medication group decreased compared to 1 day of medication,with a statistically significant difference(P<0.01).The magnitude of the decrease was as follows: sodium iodide group>iodine rich traditional Chinese medicine group>iodine rich traditional Chinese medicine compound group.5.RT-PCR measurement of TPO m RNA results: The levels of TPO m RNA in the iodine rich traditional Chinese medicine compound group and sodium iodide group increased compared to the control group after 1 day,with statistically significant differences(P<0.05 in the iodine rich traditional Chinese medicine compound 1-day group;P<0.01 in the sodium iodide 1-day group).There was no statistically significant difference in TPO m RNA levels between each medication group after 6 days of medication and 1 day of medication,P>0.05.6.Western blot analysis of NIS protein expression: After 1 day of medication,the sodium iodide group showed a statistically significant decrease in NIS protein expression compared to the control group,P<0.01;There was no statistically significant difference in the expression level of NIS protein between the other two medication groups and the control group after 1day of medication;After 6 days of medication,compared to 1 day of medication,the expression level of NIS protein in each medication group decreased,with a decrease of:sodium iodide group>iodine rich traditional Chinese medicine group>iodine rich traditional Chinese medicine compound group.7.Western blot analysis of TPO protein expression level: The iodine rich Chinese medicine group and sodium iodide group showed a statistically significant decrease in TPO protein expression level compared to the control group after 1 day of medication,P<0.01.The iodine rich Chinese medicine group showed a statistically significant decrease in TPO protein expression level after 6 days of medication compared to 1 day of medication,P<0.01.Conclusion:Compared with the same dose of sodium iodide,the iodine rich traditional Chinese m edicine compound has a longer inhibitory effect on iodine escape,which may be relat ed to its ability to significantly increase the expression of NIS. |
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