Study On The Mechanism Of Gallic Acid-induced Apoptosis Of Melanoma Cell

Objective: Melanoma is the most deadly form of skin cancer in the world,and its global burden continues to rise.More and more evidence shows that natural medicines have a significant inhibitory effect on melanoma.Therefore,this study aims to verify the effect of natural compound Gallic Acid(GA)on melanoma and explore its potential mechanism.Methods: Melanoma cell lines B16-F10 and A375 were used in this study.The effects of GA on the growth of B16-F10 and A375 cells were detected by MTT and plate cloning.ROS level in cell was measured by ROS Assay Kit.Cell survival rate was detected by CCK-8.Cell motility was measured by Transwell assay.Cell apoptosis was detected by flow cytometry.Apoptotic morphology was detected by Hoechst 33258 fluorescence staining.Mitochondrial membrane potential was detected by JC-1 kit.Western blot was used to analyze the expression of related proteins.Results: GA inhibited the growth of melanoma cells in a dose-dependent manner.In addition,the intracellular ROS level in GA group increased,while cell survival rate,migration and invasion ability decreased,and apoptosis level increased.At the same time,the Jak2/Stat3 signaling pathway was blocked,the mitochondrial membrane potential collapsed,and the endogenous apoptotic pathway was activated.After pretreatment with the antioxidant NAC,the effect of GA on melanoma was significantly antagonized.Conclusion: GA inhibits the proliferation,migration and invasion of melanoma and promotes apoptosis by inducing ROS accumulation to inhibit Jak2/Stat3 pathway and activate mitochondrial pathway.