| Siniperca schezeri belongs to the family Sinipercidae,and the genus Siniperca is located in the upper reaches of the Yangtze River and the Pearl River in Guizhou.Its germplasm resources are well preserved without breeding interference,but the germplasm identification and genetic research of the wild population of Siniperca schezeri is seriously lagging behind.Microsatellite markers are widely used in population genetics research because of their abundant polymorphisms,which can better reveal the genetic structure of species.To this end,based on transcriptome technology,this study developed polymorphic microsatellite markers related to the growth of S.chinensis,and analyzed the genetic structure and diversity of the main wild populations,so as to enrich the genetic background data of the wild population of S.Protection and development and utilization provide theoretical basis.Using high-throughput sequencing technology,30 wild Siniperca scherzeri from10 sites in 8 rivers were sequenced,sequenced and assembled.Unigene library alignment and gene annotation were performed.The length of the high-throughput sequencing sequence was 243.52 Gb,and 815,818,317 clean reads were obtained.After de novo assembly and de-redundancy,59,931 Unigenes were obtained.A large number of sequences containing microsatellite fragments were obtained and analyzed for their characteristics.The results showed that the frequency of at least one SSR marker per3.3 kb in the SSR data in the S.In the secondary classification entries,342 genes related to growth traits were found,and EST-SSR loci were found for 143 genes.90 loci were randomly selected from 115 loci,and a total of 189 pairs of primers were designed,of which 131 pairs The primers can amplify the product in the Siniperca schezeri,and the amplification efficiency is 69.3%.Seven microsatellite loci showed polymorphism after scanning,and the rest showed monomorphism.Using seven microsatellite molecular markers related to growth traits screened in this study and three microsatellite markers selected by other scholars,the genetic diversity of wild Siniperca scherzeri populations in seven rivers in Guizhou was studied.A total of 176 alleles were observed at 10 highly polymorphic microsatellite loci in 231 wild Siniperca scherzeri samples,and each locus had an average of 2.514 alleles.The observed heterozygosity(Ho)was 0.000-0.466,the mean observed heterozygosity was0.265.The expected heterozygosity(He)ranged from 0.227 to 0.466,and the average expected heterozygosity was 0.383.The Shannon information index(I)ranged from0.383 to 0.860,with an average of 0.627,indicating rich genetic diversity and high selection potential.The genetic diversity of the seven populations ranged from 0.032 to0.127,with weak differentiation between Songtao and Sinan,Tongren,Zhenyuan and Jianhe and between Tongren and Zhenyuan,Jianhe and Congjiang,and moderate differentiation among the remaining populations.The UPGMA clustering tree and genetic structure analysis showed that there was no strict phylogeographic relationship among the seven spotted Mandarin populations,and there was genetic admixture and serious gene penetration.The present study enriches the genetic background information of the spotted Mandarin population,which is necessary to improve the management of the wild resources of spotted Mandarin and avoid population degradation,establish a perfect population genetic genealogy and strengthen population genetic monitoring. |
PDF Full Text Request