Research On Elasticity Changes During Blood Coagulation Based On Vibration Method

In the normal human body,coagulation,anticoagulation and fibrinolysis are dynamically balanced systems that maintain the normal flow state of blood and stabilize the internal environment of human tissues.Dynamic imbalances in the blood system can lead to blood-related diseases such as thrombophilia,cardiovascular disease and coagulation factor deficiencies,and coagulation testing is an important tool for physicians to diagnose patients.Human coagulation is a process regulated by platelets and a variety of coagulation factors.The traditional four coagulation tests use plasma lacking platelets,which can only detect some of the coagulation factors and cannot study the role of platelets in the coagulation process,and cannot reveal the full picture of coagulation.Several commonly used thromboelastography assays use whole blood for testing,which can comprehensively and dynamically observe the entire coagulation process,but these assays all suffer from high costs,relatively complex mechanical structures,and processing difficulties.In view of the limitations of these assays,this thesis proposes a new strategy for studying the coagulation process using the vibration method.In this thesis,the detection principles of several whole blood coagulation analyzers were explained in detail,the changes of blood viscoelasticity during coagulation were analyzed theoretically,and a high shear rate vibration method was proposed for the detection of the whole coagulation process in combination with the platelet function analyzer(SONOCLOT).The force analysis of the change of the resistance to the probe vibration was carried out,and the corresponding mathematical model was established to derive the relationship between the resistance to the probe and the blood modulus;then finite element simulation was carried out based on this vibration force model to verify the feasibility of the subject from the simulation point of view;finally,the actual structure of high shear rate vibration was realized by using voice coil motor,the PCB board was designed and fabricated,and the corresponding experimental setup was built and tested for quality control products.The experimental results show that the experimental parameters tested by this device meet the expectations;the coagulation curves obtained from the experiments of this subject are more consistent with the normal coagulation curves of SONOCLOT.The coefficient of variation values of ACT value,MCS value,CR value and TP value obtained from the repeatability experiment were all controlled within 10%,and the reliability and repeatability of the experimental data met the requirements,which could reflect the changes of elasticity in the blood coagulation process,and initially proved the feasibility of the vibration method for the detection of blood coagulation process.Finally,the experimental results were analyzed for consistency with the normal reference values of SONOCLOT,which proved the feasibility of mutual substitution between the vibration method and SONOCLOT detection method proposed in this thesis.