| 【Objective】To detect the relative expression of tRF-36-PNR8YP9LON4VN1B in the plasma of patients with acute pancreatitis and healthy volunteers,and to explore its potential clinical application in the diagnosis of acute pancreatitis in a preliminary manner.【Method】50 patients with acute pancreatitis attending the Department of Gastroenterology of Kunming Yan’an Hospital from July 2021 to September 2022were collected as study subjects,and another 20 healthy volunteers who were physically examined in our hospital were selected as the control group.Among them,21 patients with moderate-severe acute pancreatitis were unified as the severe(SAP)group(10 males,11 females,median age:42 years),29 patients with mild acute pancreatitis were included as the mild(MAP)group(15 males,14 females,median age:39 years),and 20 healthy individuals were included as the control group(9 males,11 females,median age:40 years).The relative expression of tRF-36-PNR8YP9LON4VN1B in the plasma of the three groups of subjects was measured by real-time fluorescence quantitative polymerase chain reaction(RT-q PCR)technique.Statistical analysis of the expression of tRF-36-PNR8YP9LON4VN1B in the plasma of patients with acute pancreatitis and its potential as a biomarker for the diagnosis of acute pancreatitis and its clinical value for the early assessment of the severity of acute pancreatitis was performed.The measurement data were expressed as mean±standard deviation(Mean±SD)or median,and the count data between groups were tested using theχ~2test.The analysis of whether the measurement data conformed to normal distribution,the comparison between two groups of measurement data or rank data that did not conform to normal distribution was performed by rank sum test,the comparison between two groups of measurement data that conformed to normal distribution was performed by t-test of two samples in a group,the comparison between multiple groups was performed by Analysis of Variance,and the comparison between groups was performed by Least Significant Difference method;the correlation analysis between variables was performed by linear correlation,using binary logistic regression model independent risk factors for acute pancreatitis were screened.The expression levels of tRF-36-PNR8YP9LON4VN1B in each group and the correlation with the clinical indicators of patients with acute pancreatitis in each group were analyzed according to the statistical results,while ROC curves were plotted to analyze its diagnostic value in acute pancreatitis and the efficacy of each clinical indicator in early prediction of the severity of acute pancreatitis.P<0.05 was considered statistically significant.【Results】1.There was no significant correlation between gender(χ~2=0.325,P=0.746),age(χ~2=0.643,P=0.524),C reactive protein(Z=-1.763,P=0.078),white blood cells(t=0.095,P=0.925),amylase(t=-1.675,P=0.100),and triglycerides(Z=-0.541,P=0.598)between the mild and severe groups.Differences in calcitoninogen(t=-2.668,P=0.015),interleukin-6(t=-2.405,P=0.020)and blood calcium(t=4.225,P<0.001)were statistically significant(P<0.05)in both the mild and severe disease groups.2.The relative expression of tRF-36-PNR8YP9LON4VN1B was 0.69±0.34 in the healthy control group,1.44±0.62 in the mild disease group and 2.72±1.92 in the severe disease group(P<0.05).Multisample Analysis of Variance and comparison between groups showed that the overall means of relative expression of tRF-36-PNR8YP9LON4VN1B in the three groups were statistically different(F=22.59,P<0.001),plasma tRF-36-PNR8YP9LON4VN1B expression levels in the control group were statistically different from those in the mild disease group(P=0.027),plasma tRF-36-PNR8YP9LON4VN1B expression levels in the control group were statistically different from those in the severe disease group(P<0.001),and plasma tRF-36-PNR8YP9LON4VN1B expression levels in the mild disease group were statistically different from those in the severe disease group(P<0.001).3.Logistic regression results showed that after adjusting for confounders,there was a high and statistically significant association between tRF-36-PNR8YP9LON4VN1B and the risk of developing acute pancreatitis(OR=35.15,95%CI 5.44-227.16,P<0.01),and the association between both sex(P=0.56)and age(P=0.36)and The association between the two and the risk of developing acute pancreatitis was not statistically significant.4.tRF-36-PNR8YP9LON4VN1B expression levels were positively correlated with Ranson score,BISAP score,APACHEII score,MCISI score in patients with acute pancreatitis,with R~2of 0.314,0.273,0.372,0.328(P<0.001),and with PCT(r=0.38,P=0.006),IL-6(r=0.43,P=0.002)were respectively positively correlated(P<0.05)and with Ca(r=-0.074,P=0.607)were not correlated(P>0.05).5.The area under the curve of peripheral tRF-36-PNR8YP9LON4VN1B to diag-nose acute pancreatitis was 0.897,where the optimal tRF-36-PNR8YP9LON4VN1B concentration cut-off value for predicting acute pancreatitis was 1.25,the se-nsitivity was 70%and the specificity was 95%.6.tRF-36-PNR8YP9LON4VN1B,PCT and IL-6 combined for the diagnosis of acute severe pancreatitis:area under the curve was 0.787,95%confidence interval was0.656-0.917,the sensitivity was 81%and the specificity was 69%.【Conclusion】1.tRF-36-PNR8YP9LON4VN1B is upregulated in the plasma of patients with acute pancreatitis,it has some utility in the diagnosis of acute pancreatitis and it may be a potential plasma biomarker for the diagnosis of acute pancreatitis.2.tRF-36-PNR8YP9LON4VN1B expression level was positively correlated with the severity of acute pancreatitis.3.The combination of tRF-36-PNR8YP9LON4VN1B,PCT and IL-6 in peripheral blood has some predictive value for the occurrence and progression of severe acute pancreatitis. |
