Detection Of Helicobacter Pylori Nucleic Acid And Antigen In Vaginal Candida Of Pregnant Women And Neonatal Gastric Juice

Objective:To know whether there is Helicobacter pylori(H.pylori)exists in vaginal Candida of pregnant women and whether H.pylori infection occur in the neonatal period.Methods:Serum and vaginal secretions were collected from 50 randomly included pregnant women who were positive for Candida vaginalis during routine obstetric examination from the obstetric outpatient clinic of Jinyang Hospital.Maternal sera were tested for H.pylori-specific antibodies,vaginal secretion specimens were isolated and identified for Candida,and total vaginal secretion DNA was extracted for H.pylori-specific 16S rRNA gene testing.The H.pylori-specific 16S rRNA gene in Candida was tested using PCR.For samples positive for the H.pylori-specific 16S rRNA gene in Candida,the cagA virulence gene was tested.Bacterium-like bodies within Candida cells were observed by optical microscopy.The H.pylori antigen within Candida was detected using an immunofluorescence assay.Thirty newborns who underwent gastric fluid testing at the Laboratory Department of the Second People’s Hospital of Guiyang(Jinyang Hospital)were enrolled and their gastric juice was collected and tested for H.pylori specific antigen by colloidal gold assay.Five were followed up after one year and their stools H.pylori antigen was detected via the colloidal gold method.Results:1.Among 50 pregnant women,12(24%)were positive for H.pylori antibody in serum.90%of sample Candida were Candida albicans.Among 50 vaginal secretion samples,10%of the vaginal secretions were positive for H.pylori 16S rRNA gene.The H.pylori 16S rRNA gene was positive in Candida from pregnant women who was also positive for serum,among them 5 strains of Candida cagA gene were positive,while the other 38 strains of Candida H.pylori 16S rRNA gene was negative;.Among 12 strains of Candida which H.pylori 16S rRNA gene was positive,8 strains(66.7%)shows that bacterium-like bodies within Candida and the H.pylori antigen within Candida was detected using an immunofluorescence assay.2.Among 30 samples of neonatal gastric juice,17(57%)were positive for H.pylori antigen;The stool H.pylori antigen showed that 2 patients H.pylori antigen in stool was positive in of the 3 patients with positive for H.pylori antigen in gastric juice;H.pylori antigen in stool of 2 patients with negative for H.pylori antigen in gastric juice was negative.Conclusions:H.pylori nucleic acid and antigen were detected within vaginal Candida constituting a risk for transmission through childbirth.H.pylori infection may occur in the neonatal period.