Research On Neural Circuitry Mechanism Of Increased Repetitive Restricted Behaviors In Pax2 Gene Neuron-Specific Deletion Mice

Objective: With the Pax2 gene neuron-specific knockout mice(Nestin-Pax2)as subjects in the dissertation,tracer virus technique was adopted to study the mechanism of their neural circuits,and RNA sequencing technique was employed for transcriptome analysis in the hippocampus of Nestin-Pax2 mice.The study provides new research ideas on the pathogenesis mechanisms of repetitive restricted behaviors.Methods: 1.Four mice in each group were randomly selected respectively from the experimental group(Nestin-Pax2 mice)and the control group(Pax2 flox mice).With stereotactic injection of cross-multistage synaptic tracer virus HSV-EGFP(200n L/piece)into the media prefrontal cortex,cardiac perfusion,brain extraction and frozen sections were performed after 3 days so as to observe the brain region of fluorescence transmission.2.Total RNA was extracted from the hippocampal tissues of the brains of mice in the experimental and control groups(3 mice in each group),and m RNA was enriched by magnetic beads.The c DNA library was constructed by reverse transcription and sequenced using an Illumina Hi Seq4000 sequencer.3.The sequencing results were filtered in order to obtain the final data available.The data from the experimental and control group were compared so that the differentially expressed genes were screened and then performed by GO functional enrichment analysis.Results:1.The number of neurons in the m PFC-CA1-BLA neural circuitry of Nestin-Pax2 mice increased significantly by comparison with the control group.2.Gene expression in the hippocampal region of Nestin-Pax2 mice was upregulated by 85 and downregulated by 96 compared to controls.3.Compared to controls,The GO functional enrichment indicated the differences in neurotrophic factors such as insulin-like growth factor binding protein-2(IGFBP2)in the hippocampus of Nestin-Pax2 mice.Conclusion: 1.Pax2 gene neuron-specific deletion leads to changes in m PFC neural circuitry.2.Pax2 gene neuron-specific deletion leads to changes in gene expression in the hippocampus.3.The increase of repetitive restricted behaviors in Nestin-Pax2 mice may be related to the increase of IGFBP2 in hippocampus.