| Part 1. Observation on the behavior of VPA ratsMethod:The model of autism was established by the intraperitoneal injection of sodium valproate(600mg/Kg) on the 12.5 day of Pregnant rats. Then,there are a list of development and social behavior test to assess the model puppy comparing with the normal control groups.Results:Comprised to control group,the offspring in model group showed lesser of the weight(P<0.05), delayer of eyes opening(P<0.05), swimming behavior was significantly delayed(P<0.05).And significantly increased locomotor and repetitive behavior(P<0.05).The frequency of standing and crossing cell in model group is less than control group in open field test(P<0.05). Olfactory discrimination test revealed longer latency to reach home bedding in VPA group on PNDs 9 and 10(P<0.01), with no difference on PND 11Part2.To study the improvement of repetitive behavior in autism rat using essential oil of Vanillin through olfactory pathway and its possible mechanism.Methods:Tweenty-four autism animals were randomly as control group and vanillin group,while the saline group rats as normal control group and normal blank group. To observe the repetitive behavioral changes at different time and the concentration of 5-HT in the rat’s amygdala after the treatment.Result:Comprised the control groups,the repetitive behaviors of vanillin group had decreased(p<0.01) and the expression of 5-HT in Amygdala had increased after the treatment.conclusions:1. Much more complicated and typical clinical symptoms of autism have been found in the offspring of SD rats that prenatal rats’ exposure to valproate2. Vanillin by Olfactory pathway can improve repetitive behaviors of autism rats,its possible mechanism was the increasing expression of 5-HT in Amygdala,then affecting the expression of neurotrophic factors which can regulate nerve regeneration,and causing the brain function adjustments and regions remodeling. |
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