| Objective:The minichromosome maintenance complex component 6(MCM6)plays vital role in the genome replication and thus is involved in cancer initiation and progression.However,its specific function and underlying mechanisms in pancreatic cancer(PC)remains elusive.The purpose of the present research was to explore the potential prognostic value and underlying mechanisms of MCM6 in pancreatic cancer.Methods:This study determined the association between MCM6 expression and clinicopathological features and prognosis of PC by using the Encyclopedia of Cancer Cell Lines(CCLE)to study MCM6 expression levels in PC.Then,a series of in vitro experiments were conducted to investigate the role of MCM6 in the proliferation of PC cancer cells.Finally,the molecular mechanism of the link between MCM6 and PCNA(proliferating nuclear antigen)was investigated by using public database analysis and immunohistochemical staining.Results:MCM6 expression levels were significantly increased in PC cell lines and PC patients and were associated with tumor stage.Increased expression levels of MCM6 are significantly associated with poorer DFS(disease-free survival)in patients with PC.Furthermore,MCM6 ablation via sh RNA effectively inhibited the proliferative abilities of PC cells in vitro.We also found the connection between MCM6 and PCNA,which may be the molecular mechanism contributed to the effect of MCM6-mediated PC proliferation and progression.Conclusion:The present study explored the function and clinical significance of MCM6 in PC.The present study demonstrated that MCM6 can be used as a potential marker of PC and provided novel evidence.Thus,evaluation of MCM6 should be considered in PC risk stratification and management. |
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