| Objective:Edaravone(EDA)is an antioxidant that has been reported in literature to have a protective effect on tissues and organs during ischemia-reperfusion(I/R)injury.This article discussed whether EDA can protect retinal morphology and retinal ganglion cell(RGC)in retinal ischemia-reperfusion injury in rats,and whether it can achieve its protective effect through antioxidant stress.Methods:56 healthy male SD rats were randomly divided into four groups: normal control group(NCG group),sham surgery group(SHAM group),retinal ischemia-reperfusion injury group(I/R group),and retinal ischemia-reperfusion injury+EDA group(I/R+EDA group).The NCG group did not receive any treatment,while the SHAM group only underwent anterior chamber puncture after anesthesia.The I/R group and I/R+EDA group established retinal ischemia-reperfusion injury models using anterior chamber perfusion method,with intraocular pressure rising to 110 mm Hg for 60 minutes.After successful modeling in the I/R+EDA group,Edaravone 10mg/kg was intravenously administered.24 hours after surgery,the rats were euthanized and their retinas were stained with HE and TUNEL.The changes in retinal structure,full retinal thickness,and RGC survival were observed under an optical microscope in each group of rats;The activity of superoxide dismutase(SOD)in retina of each group was detected by xanthine oxidase method,the content of malondialdehyde(MDA)in retina of each group was detected by thiobarbituric acid colorimetry,and the inflammatory factor IL-1β,18 in retina of each group was detected by ELISA The content of Nrf2,HO-1,Bcl-2,and Bax protein expression in the retina of each group were detected by Western blot method.Results:1.HE staining: 24 hours after surgery,there was no significant difference in the full retinal thickness between the SHAM group and the NCG group(P>0.05).In the I/R group and the I/R+EDA group,the cells in the retinal ganglion cell layer were disordered,and the full retinal thickness was thickened(P<0.05),but the full retinal thickness in the I/R+EDA group was smaller than that in the I/R group(P<0.05).2.TUNEL staining: There was no statistically significant difference in RGC count and apoptosis rate between the SHAM group and the NCG group 24 hours after surgery(P>0.05).Compared to the NCG group,the I/R group and I/R+EDA group had a decrease in RGC count and an increase in apoptosis rate.However,the I/R+EDA group had an increase in RGC count and a decrease in apoptosis rate compared to the I/R group(P<0.05).3.SOD activity and MDA content: There was no statistically significant difference in SOD activity and MDA content in rat retinal tissue between the SHAM group and the NCG group 24 hours after surgery(P>0.05).Compared with the NCG group,the I/R group and the I/R+EDA group decreased SOD activity and increased MDA content in rat retinal tissue(P<0.05),while the I/R+EDA group increased SOD activity and decreased MDA content compared to the I/R group(P<0.05).4.IL-1 βAnd IL-18 levels: Compared with the NCG group,the SHAM group had IL-1 in rat retinal tissue 24 hours after surgery β、 There was no statistically significant difference in IL-18levels(P>0.05).Compared to the NCG group,the I/R group and the I/R+EDA group had IL-1in the retinal tissue of rats β、The level of IL-18 increased(P<0.05),and compared with the I/R group,the I/R+EDA group had IL-1 in rat retinal tissue β、The level of IL-18 decreased(P<0.05).5.Nrf2 and OH-1 protein expression: There was no statistically significant difference in Nrf2 and OH-1 protein expression between the SHAM group and the NCG group 24 hours after surgery(P>0.05).Compared with the NCG group,the Nrf2 and OH-1protein expression in the retina of the I/R group and I/R+EDA group decreased(P<0.05),while the Nrf2 and OH-1 protein expression in the retina of the I/R+EDA group increased(P<0.05).6.BCL-2 and Bax protein expression: There was no statistically significant difference in the expression of BCL-2 and Bax protein in rat retina tissue between the SHAM group and the NCG group 24 hours after surgery(P>0.05).Compared with the NCG group,the expression of BCL-2 protein in the retina of the I/R group and the I/R+EDA group was significantly reduced,while the expression of Bax protein was significantly increased(P<0.05).The expression of BCL-2 protein in the retina of the I/R+EDA group was significantly increased,while the expression of Bax protein was significantly reduced(P<0.05).Conclusions: 1.Establishing a rat retinal I/R injury model by increasing anterior chamber pressure does not affect the retinal tissue structure and related research indicators in rats.2.Retinal I/R injury is caused by oxidative stress,inflammatory factors,and cell apoptosis.3.EDA plays a protective role in retinal I/R injury in rats,and its mechanism may be to activate the Nrf2/HO-1 signaling pathway,thereby exerting anti-inflammatory,antioxidant,and anti apoptotic effects on the retina. |
