Association Of Gallbladder Cholesterol Stones With SR-B1 Single Nucleotide Polymorphism And Environmental Factors

Objective: A clinical case-control study was conducted to explore the expression and genetic polymorphism of SR-B1 gene(rs5888 and rs838880)in patients with cholesterol gallstone,and to analyze the difference and correlation between SR-B1 gene locus and blood lipid index,sex,nationality and environmental factors in patients with cholesterol gallstone.Methods: A total of 200 patients who were admitted to our department for elective laparoscopic cholecystectomy were randomly selected.According to the admission criteria,the gallbladder cholesterol stone group(100 cases)was taken as the case group.DNA was extracted from peripheral venous blood of all subjects in non-cholecystolithiasis group.Direct sequencing was used to detect SR-B1 gene(rs5888,rs838880)SNP typing in case group and control group.The differences of genotypic frequency and allele frequency of SR-B1 gene(rs5888,rs838880)in the two groups were compared to explore whether there was any association between the two groups.At the same time,the relationship between SR-B1 gene locus and blood lipid index,sex,nationality and environmental factors in patients with cholesterol gallstone was analyzed.Results: 1.The levels of serum triglyceride(TG),total cholesterol(TCH),low density lipoprotein(LDL)and apolipoprotein B(Apo-B)in the case group were significantly higher than those in the control group,while the levels of high density lipoprotein(HDL)and apolipoprotein AI(Apo-AI)in the case group were significantly lower than those in the control group.There were significant differences in allele frequency and genotype frequency of 2.SR-B1 gene rs5888 locus between the case group and the control group(P <0.05),and there were significant differences in allele C and T frequencies between the two groups.In the case group,the CC genotype of SR-B1 gene rs5888 locus was significantly higher than that of CT+TT genotype,indicating that the mutant genotype CC may be a risk factor for cholesterol gallstone(OR=2.279).The frequency of C allele is higher than that of T allele,which indicates that C allele may increase the risk of cholesterol gallstone(OR=1.898).The gene frequency of TT type is significantly lower than that of control group.HDL-C of TT type is significantly higher than that of CC and CT type,while the content of TG and TCH is significantly lower than that of CC and CT type.There was no significant difference in LDL,Apo-AI,Apo-B and Apo-AI/Apo-B.There was significant difference in the distribution of allele frequency and genotype frequency of 3.SR-B1 gene rs838880 between the case group and the control group.The CC genotype of SR-B1 gene rs838880 locus in the case group was significantly less than that in the control group,which indicated that CC,the genotype of rs838880 locus of SR-B1 gene,was a protective genotype.In the case group,the TCH of TT genotypes was significantly higher than that of TC and CC genotypes.In this group,the HDL of CC genotype was significantly higher than that of TC and TT genotype.This suggests that the C allele may be related to the increase of HDL-C in blood.There was no significant difference in TG,LDL,Apo-AI and Apo-B.4.Having a family history of cholesterol(OR=63.623)and eating desserts(OR=40.405)were risk factors for cholesterol gallstones,while tea drinking habits(OR=0.000),exercise habits(OR=0.001)and eating and breakfast habits(OR=0.001)were protective factors for cholesterol stones.5.Patients with CC genotype(SR-B1 gene rs5888)are more likely to develop cholesterol gallstone(OR=1.335)than those without a family history of cholesterol gallstone.Patients with tea drinking habit and genotype(SR-B1 gene rs5888)CC decreased risk by 84.7%(OR=0.153)compared with patients with no tea drinking habit and genotype(SR-B1 gene rs5888)CC genotype.Patients with CC genotype(SR-B1 gene rs5888)who had breakfast habits had a 49.6% lower risk than those without breakfast habits(OR=0.504)(all P <0.05).Patients with CC genotype(SRB1 gene rs838880)are more likely to develop cholesterol gallstones(OR=9.765)than those with no tea drinking habits.Patients with exercise habits and CC genotype(SR-B1 gene rs838880)had a higher risk of disease(OR=48.000)than those with no exercise habits and CC genotype(SR-B1 gene rs838880).Patients with TC and TT genotypes(SR-B1 gene rs838880)had a 30.0% higher risk of eating sweets than those who did not like sweets(OR=1.300).For patients with TC and TT genotypes(SR-B1 gene rs838880),those with breakfast habits had a 21.4% lower risk than those without breakfast habits(OR=0.786).Conclusion: 1.The CC genotype of SR-B1 gene rs5888 may be a risk genotype for cholester ol gallstone.2.The CC genotype of SR-B1 gene rs838880 may be the protective genotype of cholesterol gallstone,and the C allele may be related to the increase of blood HDL-C.3.The s ingle nucleotide polymorphism of SR-B1 gene is significantly associated with abnormal blood lipid and lipoprotein metabolism.4.Patients with cholesterol gallstones have different degree s of abnormal metabolism of blood lipids and lipoproteins.5.Having a family history of chole sterol gallstone and eating desserts are the risk factors of cholesterol gallstone,while tea drink ing habit,exercise habit and breakfast habit are the protective factors of cholesterol gallstone.6.The interaction between genetic factors and environmental factors promotes the occurrence of cholesterol gallstones.