Effects Of Long Non-Coding RNA H19 On Endometrial Receptivity And Clinical Significance

Objective: Endometrial receptivity is essential for embryo implantation and is regulated by several factors and genes,among others.Among them,the role of lnc RNA in embryo implantation has attracted increasing attention.lnc RNAs are a class of RNAs that have no protein-coding function,are more than 200 nucleotides in length,and are involved in the regulation of various physiological and disease processes.In this study,human endometrial cancer Ishikawa cells were transfected by knocking down and overexpressing long non-coding RNA(lnc RNA).To investigate the effect of lnc RNA on endometrial receptivity and its clinical significance,and to provide reference ideas for embryo implantation.Methods: 1.Human endometrial cancer Ishikawa cells were routinely cultured.The experiments were divided into Control,silnc RNA H19-NC,silnc RNA H19,lnc RNA H19-NC,and lnc RNA H19.Firstly q RT-PCR was performed to detect the expression of lnc RNA H19 in the interference group,and the sequence with a better interference effect was selected for subsequent experiments.2.CCK8 detects the proliferation ability of Ishikawa cells.Transwell assay of Ishikawa cell migration ability.The expression of lnc RNA H19,HOXA10,and integrin β3 in Ishikawa cells was detected by q RT-PCR.To analyze whether knockdown and overexpression of lnc RNA H19 affect the expression of HOXA10 and integrin β3,and to further analyze the relationship between lnc RNA H19 and endometrial receptivity.3.Estrogen treatment of Ishikawa cells was divided into three groups,0h,24 h,and 48 h.q RT-PCR was performed to detect the expression of lnc RNA H19 and analyze whether the length of estrogen treatment affected lnc RNA H19.Results: 1.Compared to the control group,the interference effect of silnc RNA H19-2was better among the three sequences,and this interference sequence was selected for the follow-up experiments.2.qRT-PCR detected the expression of lnc RNA H19,HOXA10,and integrin β3 in Ishikawa cells in each group.Compared to the control group,in the lnc RNA H19 overexpression group,the expression of lnc RNA H19,HOXA10,and integrin β3 was increased,and the differences were statistically significant(P < 0.05);in the silnc RNA H19 interference group,the expression of lnc RNA H19,HOXA10,and integrin β3 was decreased,and the differences were statistically significant(P < 0.05).3.CCK8 detects the proliferation ability of Ishikawa cells.Compared to the control group,the proliferation ability of Ishikawa cells was enhanced in the lnc RNA H19 overexpression group,and the difference was statistically significant(P < 0.05);in the silnc RNA H19 interference group,the proliferation ability of Ishikawa cells was diminished,and the difference was statistically significant(P < 0.05).4.Transwell assay to detect the effect of lnc RNA H19 on the migration ability of Ishikawa.Compared to the control group,the migration ability of the lnc RNA H19 overexpression group was enhanced,and the difference was statistically significant(P< 0.01).The migration ability was reduced in the silnc RNA H19 interference group,and the difference was statistically significant(P < 0.05).5.The expression of lnc RNA H19 was higher in estrogen-treated Ishikawa cells for 48 h.The difference was statistically significant(P < 0.05).Conclusion: Knockdown and overexpression of lnc RNA H19 affect the expression of HOXA10 and integrin β3,which in turn affects endometrial receptivity.In addition,estrogen treatment of Ishikawa was found to promote the expression of lnc RNA H19.It was demonstrated that estrogen has a regulatory effect on endometrial receptivity,and the exact mechanism needs to be further investigated.This paper provides ideas for studying endometrial receptivity and provides a reference for improving embryo implantation rates.Figure[12] Table[8] Reference[67]...