| Objective:To explore the study of rinsing conjunctival sac with different frequency of povidone-iodine(PVI)on ocularsurface damage,andto provide a scientific evidences for the prevention and cure of ocular surface injury caused by repeated use of povidone-iodine in clinic.Methods:Twelve SD rats were randomly divided into four groups,the control group received normal saline irrigation of conjunctival sac,the experimental group was divided into three groups,with the left eye as the experimental eye and the right eye as the blank control eye,respectively receiving 0.5%PVI(Kangweida,Hangzhou Xizi)irrigation of conjunctival sac once,twice and three times,and receiving normal saline irrigation again after 90 s.The remaining PVI in the conjunctiva capsule was rinsed,and the interval between two PVI rinses was 28 days.In the control group before normal saline irrigation of conjunctival sac(D0),the experimental group before the last PVI irrigation of conjunctival sac(D0),the first day after irrigation(D1),the seventh day after irrigation(D7),the fourteenth day after irrigation(D14),the 28 th day after irrigation(D28)five periods,The tear film rupture time(BUT),tear secretion volume,corneal fluorescein staining score and ocular surface inflammation index score of the four groups of rats were observed to evaluate their influence on the ocular surface,and observe the recovery of the ocular surface after injury.Finally,large amounts of chloral hydrate were injected into theabdomen,and therats were killed.Results:1.The average tear secretion volume of group A was statistically significant in D1 compared with D0(P=0.02 < 0.05),but was not statistically significant in D7,D14 and D28 compared with D0(P > 0.05).The mean tear film rupture time D1,D7 and D14 were statistically significant compared with D0(all P < 0.05),while D28 was not statistically significant compared with D0(P=0.42 > 0.05),and the scores of corneal fluorescein staining score and inflammatory index score were0,sothe data werenot statistically significant.2.The average tear secretion volume in group B was statistically significant in D1 compared with D0(P=0.03 < 0.05),but was not statistically significant in D7,D14 and D28 compared with D0(P > 0.05).The mean tear film rupture time of D1,D7 and D14 was statistically significant compared with D0(all P < 0.05),but was not statistically significant compared with D28 and D0(P=0.23 > 0.05).The average corneal fluorescein staining scores D1,D7,D14,D28 were not statistically significant compared with D0(all P > 0.05).There was no significant difference in average inflammatory index scores D1,D7,D14,D28 compared with D0(all P> 0.05).3.The average tear secretion D1,D7,D14,D28 in group C was significantly different from that in group D0(all P < 0.05).The mean tear film rupture time D1,D7,D14 and D28 were statistically significant compared with D0(all P < 0.05).The average corneal fluorescein staining scores D1,D7 and D14 were statistically significant compared with D0(all P< 0.05),but were not statistically significant compared with D28(P=0.18 > 0.05).There was no significant difference in average inflammatory index scores D1,D7,D14,D28 compared with D0(all P> 0.05).4.Compared with D0,average tear secretion D1,D7,D14 and D28 in group D had statistically significant differences(all P < 0.05).The mean tear film rupture time D1,D7,D14 and D28 were statistically significant compared with D0(all P < 0.05).The average corneal fluorescein staining scores D1,D7,D14,D28 were statistically significant compared with D0(all P < 0.05).The average inflammatory index scores D1,D7,D14 and D28 were statistically significant compared with D0(all P< 0.05).5.In time period D1,there were statistically significant differences among any group in three indexes of tear secretion,tear film rupture time and corneal fluorescein staining score(all P<0.05).In the score of corneal inflammation index index,there was no statistical significance between group A and group B(P=0.14 > 0.05),while pairwise comparison of other groups showed statistically significant differences(P< 0.05).6.In time period D28,there were no statistically significant differences in the four indicators between group A and group B(all P > 0.05),but there were statistically significant differences inthefour indicators between anyother two groups(all P< 0.05).Conclusion:1.According to this study,both normal saline and PVI flushing conjunctival sac can cause certain damage to the ocular surface.Serious damage was caused to the ocular surface on the first day after irrigation.With the passage of time,the ocular surface gradually recovered,and it could return to the normal ocular surface level after the first PVI irrigation and 28 days after the normal saline irrigation.2.With the increasing frequency of PVI flushing,the damage to the ocular surface was gradually aggravated.The damage to the ocular surface of group C was more serious than that of group B,and the damage to the ocular surface of group D was more serious than that of group C.3.With the increasing frequency of PVI flushing,the recovery time of ocular surface became longer and longer.The recovery time of ocular surface in group C was significantly longer than that in group B,and the recovery time of ocular surface in group D was significantly longer than that in group C.4.It has been proved that multi-frequency PVI irrigation of conjunctival sac has additive effecton ocularsurface injury andocular surface recovery time. |
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