Relationship Between CXCR1 And CXCR2 Gene Polymorphisms And Lung Cancer Risk

Objective: Lung cancer is one of the most common cancer in the world,Both the incidence and mortality of lung cancer rank the first among tumor-related deaths and the situation is becoming increasingly severe,which seriously affects public health.In order to alleviate alleviate this situation,medical workers around the world have tried to take some proactive measures to prevent and control the occurrence and development of cancer,but the results are still not satisfactory.Many research results show that the occurrence of cancer is the result of a complex multi-stage and multi-cause interaction,including environmental factors and genetic factors,etc.Smoking and air pollution are common environmental factors,and single nucleotide polymorphism is a common genetic factor.CXCR1 and CXCR2 gene polymorphisms have shown that involved in the occurrence and development of gastric cancer,colorectal cancer,breast cancer,HIV infection,asthma,chronic obstructive pulmonary disease,systemic sclerosis and other diseases,so it is speculated that they may affect the susceptibility to lung cancer,but their different research results with lung cancer susceptibility is still controversial,Moreover,there are differences in genetic background among different populations.Therefore,the relationship between them needs to be further explored in different populations and regions.In summary,this study aimed to investigate the relationship between CXCR1 rs16858841 and CXCR2 rs1126579 single nucleotide polymorphisms and lung cancer susceptibility in Shenyang.Methods: In this study,a case-control study was used to collect data of lung cancer patients and comparable data of healthy physical examiners from multiple Grade A Third Hospital in Shenyang.According to the larger estimated sample size,a total of 476 cases of lung cancer and 513 healthy controls were enrolled.Two loci(rs16858841 and rs1126579)of all samples were genotyped by Taqman probe method and 7500 fast real-time quantitative PCR.Z test was conducted to analyze the distribution difference between the two groups for continuous variables such as age;gender,smoking status and other categorical variables were analyzed using χ2 test.The odds ratio and 95% confidence interval were calculated by Logistic regression analysis.Goodness-fit χ2 test was used to evaluate whether each genotype in the control group met Hardy-Weinberg equilibrium.The software used for statistical analysis was SPSS 25.0 and software written by Anderson.Results: No statistically significant difference was found between CXCR1 rs16858841 and CXCR2 rs1126579 polymorphisms and the risk of lung cancer in the population.At rs16858841: GA genotype were 0.710 times more likely to develop lung cancer than GG genotype;Individuals with the GA+AA genotype had a 36.2% reduced risk of developing lung cancer,and the difference were not statistically significant.rs1126579: Compared with CC genotype,Individuals with the CT genotype had a 24 percent increased risk of lung cancer;The TT genotype carrier was increased 9% more likely to develop lung cancer;The risk of lung cancer was 22.6% higher for individuals with CC genotype;TT genotype carrier was 5.6% decrease of developing lung cancer than CT+CC genotype carrier,and the difference were not statistically significant.In the stratified analysis of pathological types,no correlation was found between rs16858841 and rs1126579 and lung adenocarcinoma,lung squamous cell carcinoma,small cell lung cancer and non-small cell lung cancer.rs16858841 and rs1126579 had no statistical significance in the stratified analysis of gender status,age status and smoking status.Crossover analysis showed that compared with non-smokers with GG genotype,rs16858841 smokers with GG genotype had 2.502-fold higher risk of lung cancer(OR=2.502,95%CI=1.848-3.386,P<0.001),smokers with GA+AA genotype had a 0.835-fold lung cancer risk and the difference was not statistically significant.At rs1126579,compared with non-smokers with CC genotype,smokers with CC genotypes had a 2.117-fold increased risk of lung cancer(OR=2.117,95%CI=1.041-4.304,P=0.038);smokers with CT+TT genotypes had a 2.831 times increased risk of lung cancer(OR=2.831,95%CI=1.635-4.903,P<0.001),suggesting that there may be interaction rs16858841 polymorphisms and smoking status.However,both additive and multiplicative interaction analyses were not statistically significant.Conclusions: The result of research shows that CXCR1 rs16858841 and CXCR2rs1126579 single nucleotide polymorphisms were not significantly associated with the risk of lung cancer.However,smoking is the main risk factor of lung cancer and CXCR2rs1126579 polymorphisms and smoking may have a joint effect on increasing risk of lung cancer,that needs to be further verified by large sample study.In addition,we did not find joint effect between rs16858841 single nucleotide polymorphisms and smoking status in lung cancer risk.