Expression Of CXCR1 And CXCR2 In Triple-negative Breast Cancer

Triple-negative breast cancer(TNBC)in breast cancer by immunohistochemical staining,estrogen receptor(ER),progesterone receptor(PR)and human epidermal growth factor receptor 2(HER2)were negative expression,but it partly expresses epidermal growth factor receptors(EGFR)and cytokeratin5/6(CK5/6).Studies have shown that TNBC is ineffective in endocrine therapy and targeted therapy,which has attracted the attention of many researchers.Therefore,the current exploration and demand for new therapies are extremely urgent.Therefore,this study investigated the expression of CXCR1 and CXCR2 proteins in TNBC and its relationship with EGFR and CK5/6 proteins.ObjectiveTo investigate the expression of CXCR1 and CXCR2 in TNBC and their significance;To explore the molecular mechanism of TNBC and provide reference for the development of specific targeted drugs;The correlation between the expression of CXCR1,CXCR2,EGFR and CK5/6 protein in TNBC was analyzed.MethodsA total of 92 women with invasive breast cancer were collected from January 2010 to December 2011,28 cases adjacent normal tissues of breast cancer specimens as control group,30 cases invasive ductal carcinoma,not otherwise specified(IDC-NOS)and 34 cases TNBC specimens as experimental group.The expression of CXCR1 and CXCR2 protein was detected by immunohistochemistry EnVision method in adjacent normal tissues of breast cancer,IDC-NOS and TNBC,and the expression of EGFR and CK5/6 were detected in TNBC.In addition,the relationship between the four proteins and the clinicopathological features of patients was analyzed in TNBC,comparison of clinical pathological characteristics between IDC-NOS and TNBC two groups,the correlation of CXCR1,CXCR2,EGFR and CK5/6 four proteins in TNBC.ResultsBy using immunohistochemical technique,the expression of CXCR1 and CXCR2 protein was highly expressed in the adjacent normal tissues of breast cancer,however,IDC-NOS was moderate expressed and TNBC was low expressed.In addition,the positive expression rate of EGFR was 18 cases(52.9%)and EGFR negative expression rate was 16 cases(47.1%)in TNBC.The positive expression rate of CK5/6 was 17 cases(50%),and the negative expression rate of CK5/6 was 17 cases(50%)in TNBC.The positive expression rates of CXCR1 and CXCR2 in TNBC were 23.5%and 35.3%,respectively.The positive expression rates of CXCR1 and CXCR2 in IDC-NOS were 63.3%and 60.0%,respectively.In the adjacent normal tissues of breast cancer the positive expression rates of CXCR1 and CXCR2 were 89.3%and 92.9%,respectively.Expression of CXCR1 and CXCR2 protein?EGFR and CK5/6 protein and clinicopathological features of TNBC,respectively,was not significantly different(P>0.05).The clinicopathological features of TNBC included age,lymph node metastasis,tumor size and Scarfs-Bloom-Richardson(SBR).Comparison of clinical and pathological features between TNBC and IDC-NOS two groups,SBR was significantly different(P<0.05),and there was no significant difference with other clinicopathological features(age,lymph node metastasis,tumor size)(P>0.05).In the TNBC group study,a positive correlation was found between CXCR1 and CXCR2 protein(r=0.606,P=0.000);there was no correlation between CXCR1 and EGFR protein(r=-0.033,P=0.854);there was no correlation between CXCR1 and CK5/6 protein(r=0.139,P=0.434);there was no correlation between CXCR2 and EGFR protein(r=0.203,P=0.249);there was no correlation CXCR2 and CK5/6 protein(r=0.123,P=0.488);EGFR and CK5/6 protein were positively correlated(r=0.363,P=0.035).Conclusions1.The expression of CXCR1 and CXCR2 was strongly positive,moderate positive,negative decreasing trend in adjacent normal tissues of breast cancer,IDC-NOS and TNBC.It is suggested that the expression of CXCR1 and CXCR2 protein is closely related to the occurrence and development of breast cancer.2.Combined detection of CXCR1 and CXCR2 two proteins has not seen at home and abroad in TNBC.The expression of CXCR1 and CXCR2 protein in TNBC was low,and the expression of CXCR1 and CXCR2 protein in TNBC group was significantly positive correlation.3.There are six subtypes of TNBC,and the expression of CXCR1 and CXCR2 protein decreased,suggesting that some subtypes of PI3K/AKT/mTOR signaling pathway in TNBC may be a mutation or deletion of the key factors.TNBC as a high-risk breast cancer,the lack of effective treatment,this study may provide a new theoretical basis for clinical gene targeted therapy.