Study On The Linc00657 Upregulation Of Skp2 Expression For The Development Of Cervical Cancer Malignancy

Objective: To clarify the role of Lnc RNA Linc00657 in cervical cancer cells,and to explore whether Linc00657 can increase the expression of Skp2 and promote the malignant development of cervical cancer from the in vitro and in vitro levels,which provides a theoretical basis for the treatment of cervical cancer.Methods: The expression levels of Linc00657 in cervical epithelial H8 cells and cervical cancer Si Ha and He La cells were measured by q RT-PCR assay;The expression of Linc00657 in cervical cancer cells was increased and decreased by lentivirus transfection,respectively,and the efficacy of lentivirus transfection on Linc00657 expression was detected by q RT-PCR assay.The proliferation,migration,invasion and apoptosis of cervical cancer cells were detected by MTT,clone formation,wound healing,Transwell invasion and flow cytometry,respectively.Lnc RNA Linc00657 network was constructed by bioinformatics analysis.Lnc RNA-mi RNA-m RNA ce RNA network was used to find the target gene Skp2;To discover the regulatory relationship with Linc00657.The effect of the change of Linc00657 expression on Skp2 expression in Si Ha cells was detected by q RT-PCR experiment.By establishing the subcutaneous transplanted tumor model and lung metastasis model of cervical cancer in nude mice,the expression of Skp2 gene in tumors and lung tissues of different groups was studied by immunohistochemical method,and the RNA expression of Linc00657 and Skp2 in tumors and lung tissues of different groups was detected by q RT-PCR test.Results:(1)Linc00657 m RNA expression was higher in Si Ha and He La cells than in H8 cells(P < 0.05),and Linc00657 m RNA expression was higher in Si Ha cells than in He La cells(P < 0.05);(2)The expression of Linc00657 in cervical cancer Si Ha cells with Linc00657 overexpression was higher than that in empty vector EV,while the result of knocking down Linc00657 was opposite.(3)The proliferation rate,migration rate and invasion number of cervical cancer Si Ha cells with Linc00657 overexpression were higher than those in empty vector(EV)group,but the apoptosis rate was lower than that in EV group.(4)Bioinformatics analysis predicted the existence of Linc00657-mi R30s-Skp2 regulatory network,and there may be a regulatory relationship between Linc00657 and Skp2.(5)q RT-PCR assay showed that the expression of Skp2 in cervical cancer Si Ha cells with overexpression of Linc00657 was higher than that in EV group,while knocking down Linc00657 resulted in the opposite effects.(6)In the subcutaneous transplantation tumor model of nude mice,the tumor volume and mass,the expression of Skp2 m RNA and the positive expression area of Skp2 m RNA in the tumor tissues with the low expression of Linc00657 group was decreased,while in the lung metastasis model of nude mice,the expression of Skp2 m RNA and the positive expression area was decreased in the lung tissue with the low expression of Linc00657 group.Conclusion: The expression of Linc00657 was elevated in cervical cancer cells and overexpression of Linc00657 may promote the growth and metastasis of cervical cancer by elevating the expression of Skp2.