Analysis Of Serum Exosomes In Patients With Diaphragmatic Budd Chiari Syndrome Of Inferior Vena Cava In Northern Anhui By LncRNA Microarray

Purpose:In this study,lncRNA microarray chips were used to analyze the lncRNA and mRNA expression profiles of serum Exosomes in patients with inferior vena cava diaphragms-type Budd-Chiari syndrome(BCS)in northern Anhui,and valuable lncRNAs involved in regulating Budd-Chiari syndrome(BCS)were screened.It provides the basis for the study of the pathogenesis of the involvement of serum Exosomes in the septal Budd Chiari Syndrome of inferior vena cava,and also provides a new noninvasive biomarker for the diagnosis of BCS.Method:Firstly,5 patients with inferior vena cava diaphragm type Budd Chiari Syndrome in the vascular surgery Department of the First Affiliated Hospital of Bengbu Medical College were selected as the experimental group and 5 normal healthy adults as the control group.Serum Exosomes were extracted,observed by electron microscope and identified by WB.lncRNA microarray chip was used to detect and analyze Exosome RNA,and Genespring software was used for quantile standardization of the original data.lncRNA and mRNA differential expression profiles were statistically analyzed,and GO(gene ontology)enrichment analysis and KEGG pathway enrichment analysis were further conducted.The co-expression network of lncRNA-mRNA was constructed,and the core genes were enriched by GO and KEGG pathway.The sera of 10 BCS with inferior vena cava septum type and 5healthy controls were collected,and 4 lncrnas among the Top10 different Lncrnas were verified by RT-qPCR.SPSS software was used for statistical analysis,and the independent sample T-test was used to compare the difference between the two groups.P value < 0.05 was considered statistically significant.Results:1.The Exosomes were observed as saucer-type by electron microscopy,and were confirmed as Exosomes by WB assay.2.mRNA and lncRNA expression profiles were analyzed by lncRNA microarray chips,and 92 differential mRNAs(30 up-regulated and 62 down-regulated)and 209 differential lncRNAs(125 up-regulated and 246 down-regulated)were obtained.GO and KEGG enrichment results of lncRNA co-expressed mRNA showed that the main biological processes were stimulatory C-type lectin receptor signaling pathway.The KEGG pathway is progesterone mediated Oocyte maturation(progesterone mediated oocyte maturation)and inflammatory mediator regulation of TRP channels(Inflammatory regulation of TRP channels).3.In this study,Hub Gene-lncRNA(PSMD5,SRGAP1,DHRS9,PRKACB,DDX52)and mRNA(COX11,PLXNA3,PPARA,ARPC4-TTLL3,SFTA2,NCKAP1L)were obtained in the co-expression network.The biological processes that are enriched most by lncRNAs are stimulatory C-type lectin receptor signaling pathway and important biological processes of energy metabolism for the synthesis of ADP and NADH.The most highly enriched pathways of lncRNAs KEGG were progesterone mediated oocyte maturation(progesterone mediated oocyte maturation)and inflammatory mediator regulation of TRP channels(Inflammatory regulation of TRP channels),suggesting that lncRNA-targeted genes may affect human T cell receptor signaling pathway,apoptosis pathway and other immune response processes at the same time.Conclusions:1.In this study,lncRNA and mRNA expression profiles of Exosomes in patients with inferior vena cava septum type Budd Chiari Syndrome in northern Anhui were analyzed by lncRNA microarray chips.2.lnc-TMEM254-AS1,lnc-MTUS2-5 and lnc-XAGE1A-3 are potential serum biomarkers of inferior vena cava septum type Budd Chiari syndrome in northern Anhui.