| 【Objectives】:The aim of this project is to investigate the biological effects of tRF-Pro-CGG in mouse pancreatic cancer cells and its possible molecular mechanisms.【Methods】:The expression levels of tRF-Pro-CGG in mouse pancreatic cancer cell lines pan02 and LTPA,human pancreatic cancer cell lines Capan-2 and normal pancreatic cancer cells HPDE6-C7 were detected by qRT-PCR.The overexpression and silencing of tRF-Pro-CGG in pan02 cells of pancreatic cancer in mouse were detected by lentiviral transfection,and the overexpression and silencing effects were detected by qRT-PCR and Western blot.The effect of tRF-Pro-CGG on the growth and metastasis of pancreatic cancer cell transplants in nude mice was examined;the histopathological structure of transplanted tumors was observed by HE staining;the expression of proliferation-related protein Ki-67,metastasis-related protein E-calmodulin(cadherin),vimentin and PI3K/AKT pathway protein expression and phosphorylation in transplanted tumor tissues were examined by Western blot.【Results】:The expression of tRF-Pro-GGG in mouse pancreatic cancer cell lines pan02 and LTPA and human pancreatic cancer cell line Capan-2 was lower than that in normal pancreatic cancer cell lines HPDE6-C,and the expression of tRF-Pro-CGGG was the lowest in pan02 cells,while the expression of tRF-Pro-CGGG was relatively high in LTPA cells.Compared with the NC mimics group,the cell proliferation ability was significantly reduced in the tRF-Pro-CGG mimics group(P<0.01),cell migration(P<0.001)and invasion ability(P<0.001)were significantly reduced,the volume(P<0.01)and weight(P<0.001)of nude mice transplanted tumors were significantly reduced,and the nude mice transplanted tumor tissues showed significant Necrotic and apoptotic cells were significantly reduced in nude mice transplant tumor tissues,and the protein expression of Ki-67 and Vimentin was significantly reduced(P<0.001)while that of E-cadherin was increased(P<0.001),and the protein expression of PI3 K,P-PI3 K,AKT,and P-AKT was significantly reduced(P<0.001)in nude mice transplant tumor tissues.compared with the NC inhibitor group,the cell proliferation ability(P<0.01),cell migration(P<0.001)and invasion ability(P<0.001)were significantly higher in the tRF-Pro-GGG inhibitor group,the volume(P<0.01)and weight(P<0.01)of nude mice transplanted tumors were significantly higher,and a small amount of necrotic and apoptotic cells appeared in nude mice transplanted tumor tissues.A small number of necrotic and apoptotic cells were found in the tumor tissues of nude mice,and the protein expression of Ki-67 and Vimentin was significantly increased(P<0.001)and E-cadherin was decreased(P<0.001),and the protein expression of PI3 K,P-PI3 K,AKT and P-AKT was significantly increased(P<0.001)in the tumor tissues of nude mice.There was no significant change in the number of liver metastases in the tRF-Pro-CGG mimics group compared with the NC mimics group(P>0.05),and no significant change in the number of liver metastases in the tRF-Pro-GGG inhibitor group compared with the NC inhibitor group(P>0.05).【Conclusions】:tRF-Pro-GGG was low expressed in mouse pancreatic cancer cells,and it inhibited the proliferation,migration and invasion of mouse pancreatic cancer cells,and inhibited the growth of transplanted tumor of pancreatic cancer in nude mice.tRF-Pro-CGG may affect the progression of transplanted pancreatic cancer in nude mice by inhibiting the PI3K/ Akt signaling pathway.tRF-Pro-CGG may also inhibit the expression of Ki-67 and Vimentin proteins in pancreatic cancer grafts,thus affecting the occurrence and development of pancreatic cancer grafts in nude mice. |
