Studying The Mechanism Of MiR-103a-3p In Nude Mice Model Of Pancreatic Cancer

Objective:To investigate the effect of miR-103a-3p on the proliferation,invasion and metastasis of pancreatic cancer in nude mice,to provide a theoretical basis for understanding the mechanism of miR-103a-3p affecting the occurrence and development of pancreatic cancer,And provide a new basis and method for the biological diagnosis and molecular targeted therapy of pancreatic cancer,so as to provide a theoretical basis for the development of new pancreatic cancer drugs.Methods:(1)The low expression vector of miR-103a-3p,negative control lentivirus,was implanted with PANC-1 pancreatic cancer cell line;(3)to construct nude mice subcutaneous tumor model,pancreatic cancer cell lines were injected subcutaneously in two groups of nude mice,each group of 5;(4)to build nude mice under the capsule tumor model,Two groups of pancreatic cancer cell lines were injected into two groups of nude mice under the pancreas capsule,each group of 5;(5)The liver and lung metastases of the pancreatic cancer model were compared between the two groups.The time of tumor formation,size,weight,visual observation and continuous section HE staining were compared between the two groups;(6)HE staining and histological examination were performed.Results:(1)The cells of control-PANC-1 group and NC-PANC-1 cells were successfully obtained and The expression rate of infection was above 80%;(2)The expression of miR-103a-3p was measured by qRT-PCR.Control-PANC-1 group was used as control group.The relative expression of miR-103a-3p in NC-PANC-1 cells was(1.799 ± 0.056).However,The expression of miR-103a-3 in control-PANC-1 group was(1.002 ± 0.072).Compared with NC-PANC-1 group,the expression of miR-103a-3p in control-PANC-1 group was significantly decreased.The difference was statistically significant(P <0.01);(3)Nude mice subcutaneous tumor formation:Rice grain size nodules were seen on day 4 of NC-PANC-1 nude mice.However,On the sixth day,the grain size nodules were seen in the control-PANC-1 group of nude mice.After that,the growth of NC-PANC-1 group was relatively rapid.The average tumor weight of NC-PANC-1 group was 1.25 ± 0.16 g.However,the average tumor weight of control-PANC-1 group was 0.68 ± 0.06 g.The difference between the two groups was statistically significant(P <0.01);(4)Nude mice underwent posterior tumor metastasis of the pancreas: All nude mice showed pancreatic subcapsular nodules.two nude mice liver metastases were observed in the NC-PANC-1 group,while the control-PANC-1 group was not found in nude mice.There was no lung metastasis in all nude mice in nude mice with control-PANC-1 group.One lung transplanted tumor was found in NC-PANC-1 group;(5)Pathological examination:The expression of Dicer-1 protein in nude mice subcutaneous of control-PANC-1 group was significantly higher than that in NC-PANC-1 group.The tumor tissue was found in the pancreas of the two groups by HE staining.Hepatic metastases were found in NC-PANC-1 group by HE staining.However,No metastatic tumor was found in control-PANC-1 group.The results of immunohistochemistry showed that the expression of Dicer-1 protein in NC-PANC-1 group was lower.Conclusion:After downregulation of miR-103a-3p gene expression in PANC-1 cells,the PANC-1 cells were injected subcutaneously in nude mice and The pancreas capsule.It was found that the PANC-1 cells could block tumor growth and inhibit tumor liver and lung metastasis.