| Objective: The number of studies on organophosphate OPEs in human urine is very large and these studies cover a wide range of regions,populations and research directions.The direction of the studies is mainly based on urinary organophosphate OPE concentrations as an indicator of exposure level evaluation.Some studies focused on the differences and correlations of organophosphate OPE concentrations in homebased populations.Some studies have analyzed the trends of organophosphate OPE in human urine,age or gender.Methods:Urine OPE-related data were collected on NHANES in children aged 8-17 years from 2011–2012,2013–2014,2015–2016,and 2017–2018,including complete data on OPE metabolites,blood pressure measurements,and core covariates(Calafat2012).We determined blood pressure categories by age,sex,and height ratio systolic(SBP)and diastolic(DBP)percentiles.We established multivariate logistic regression model by natural logarithmic conversion of OPE metabolites to become a continuous variable.The statistical models were adjusted according to age,sex,race/ethnicity,PIR,BMI,serum cotinine,hematocrit,dietary calorie intake,sodium,and NHANES cycle.Grouped analyses were performed by sex,age group(children or adolescents),and overweight/obesity to identify potential corrective factors.We examined the association between OPE metabolites and hypertension by odds ratios(ORs)and corresponding95% confidence intervals(CIs).Meanwhile,restriction cubic splines(RCS)were performed at the 10 th,50th,and 90 th percentiles to evaluate the dose-response relationship between OPE metabolite levels and high BP odds carefully.Five urine OPE metabolites are quantitatively examined by quantile g-computation(Qgcomp)while increasing the OR of high BP by one quantile.The corresponding weights for each OPE metabolism are calculated.Finally,To measure the risk of exposure to each OPE metabolite and high BP,Bayesian nuclear machine regression(BKMR)model was established.Set all mixtures to their median concentrations to assess the overall effect of OPE metabolite mixtures on high BP at different quantiles.Posterior inclusion probability(PIP)is calculated to determine the relatively important OPE metabolites in the mixture.Results:1、 1340 eligible participants were selected from the NHANES database,including 678 children and 662 adolescents.The mean age of participants was 12.46 ± 2.88 years.Men 53.13% women 46.87%.The mean measurements(±SD)of systolic and diastolic blood pressure were 105.44±9.70 and 57.14±11.40(mm Hg).The median concentrations of DPHP,BDCP,BCPP,BCEPP and DBUP were 1.41 μg/g,1.59 μg/g,0.17 μg/g and0.54 μg/g.2、In addition to DBUP,unit increases in OPE metabolites are associated with high BP odds ratios.The highest(relative to lowest)quartiles of bis(1-chloro-2-propyl)phosphate(BCPP),bis-2-chloroethyl phosphate(BCEP),and di-n-butyl phosphate(DBUP)after adjusting for dependent variables were associated with odds ratios for hypertension of 1.23(95% CI: 0.83,1.83),1.27(95% CI: 0.85,1.92),and 1.01(95% CI:0.67,1.53).3、 In Qgcomp,increased quartiles of OPE metabolite mixtures were weakly associated with increased risk of hypertension(adjusted OR: 1.06,95 CI%: 0.81,1.37).4、 the results of BKMR showed a positive trend between OPE metabolite mixtures and risk of hypertension.Conclusion Higher levels of BCPP,BCEP,and DBUP are slightly associated with hypertension in children and adolescents in our country.OPE metabolite mixtures were influence on hypertension. |
