Effects Of Different Concentrations Of Hypernatriotonic Solution On Inflammation And Liver Oxidative Injury After Severe Scald In Rats

Objective Comparing different concentrations of hypertonic sodium solution(HS)and sodium glyn lactate solution(LR)in the treatment of severe scald rats,to investigate the effects of different hypertonic sodium salts(HS)on inflammation and liver oxidative injury after severe scald in ratsMethods:After feeding 176 SPF female SD rats for 1 week,According to the random number table method,the patients were randomly divided into false injury group(8),LR group(24),200 HS group(24),300 HS group(24),400 HS group(24),600 HS group(24),800 HS group(24)and 1000 HS group(24).The rats in the sham injury group were sacrificed directly after the sham injury treatment,and the rats in the LR group were established as severe scald models and treated with 4 ml/kg sodium lactate Ringer Injection(LR).After the scalding model was established,the rats in groups 200 HS,300HS,400 HS,600HS,800 HS and 1000 HS were treated with 200 mmol/L,300mmol/L,400 mmol/L,600 mmol/L,800 mmol/L and 1000,Abdominal aorta blood was taken from the rats in the sham injury group after death.After 2,8 and 24 h of fluid supplementation,8 rats in each group were sacrificed to keep abdominal aortic blood for reserve.respectively The changes of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),high mobility group protein 1(HMGB1),malondialdehyde(MDA)and superoxide dismutase(SOD)in each group were compared and observed.Results:Compared with the sham injury group,the contents of TNF-α,IL-1β,HMGB1 in blood(except 2h after injury)and MDA in liver in LR group and 6 HS group were increased to varying degrees at all time points after injury,while the activity of DAO in liver was decreased to varying degrees,and the changes in HS600 group were the least.Compared with LR group,the contents of TNF-α,IL-1β,HMGB1 in blood(except 2h after injury)and MDA in liver of HS group with different concentrations were decreased to varying degrees at all time points after injury,while the activity of DAO in liver was increased to varying degrees in a dose-dependent manner,and the changes were most significant in HS600 group.Compared with HS600 group,the contents of TNF-α,IL-1β and HMGB1 in blood(except 2h after injury)and MDA content in liver were increased to varying degrees at all time points after injury in each group,while the activity of DAO in liver was decreased to varying degrees.The changes in LR group were the most significant,and the changes in HS400 and HS800 groups similar to HS600 group were minor.The differences were statistically significant(P < 0.01 or P <0.05).Conclusion:Compared with LR,hypertonic sodium solution of different concentrations could reduce inflammation and oxidative stress injury of liver after burn by inhibiting the release of inflammatory factors such as TNF-α and IL-1β,reducing MDA accumulation and SOD consumption in liver tissue in a dose-dependent manner,and 600 mmol/L hypertonic sodium solution had the best effect.