Electrochemical Study On Adhesion Characteristics Of Breast Cancer Cells

A large number of studies have shown that one of the reasons that cancer cells are prone to metastasis is that the adhesion characteristics of cancer cells have changed compared with normal cells.At present,the methods to testing cell’s adhesion include microtubule sucking and synchronization technology methods,real-time quantitative PCR etc.,but these methods can’t accurately reflect changes in cell adhesion properties because they detect after destruction of cells or can only report the adhesion rate.We try to use electrochemical methods which will not destruction of cells to detect the electrochemical parameters during cell adhesion to the electrode surface,then analyze those parameters to construct a sensor model to evaluate the viscosity of cells.We try to cultured breast cancer cells MDA-MB-231,MCF 7 and normal breast cells MCF 10 A on ITO conductive glass electrode,and using integrin αvβ3 antibody to modify those cells,it was found that integrin αvβ3 antibody would hinder the adhesion of MDA-MB-231 cells.Based on the characteristic that tumor cells will hinder electron transfer when they adhere to the electrode surface,a sensor that can reflects intuitively changes in cell adhesion characteristics through changes in viscosity was construct.Our study consists of four mainly parts:The first part is introduction and related research introductions,mainly describing the cell electrochemical characteristics and the principle of the cell electrochemical sensor.The second part is the cultivation of breast cancer cells MDA-MB-231,MCF 7and normal breast cells MCF 10 A.Simultaneously,modification of cells with integrin antibodies and then observe the effect of integrin antibody on cell adhesion.Culture cells on ITO conductive glass and evaluate the biocompatibility of ITO conductive glass.For the third part,firstly we made a unique ITO conductive glass electrode model,then built an electrochemical detection system.We inoculated cells on ITO conductive glass electrode and the electrical impedance parameters of the ITO conductive glass electrode were detected.We find that the number of cells was positively correlated with the system impedance,and the impedance characteristics were related to cell’s type.Modification of the cells with integrin αvβ3 antibody will lead an impedance decrease in the MDA-MB-231 cells adhesion system.The fourth part is the construction of the breast cancer cell adhesion characteristic sensor,after measuring the electrochemical parameters of the standard viscosity fluid,a normalized impedance-viscosity model is established.This model can intuitively reflect the changes in the viscosity of cells during the adhesion process,and the modification of the integrin αvβ3 antibody causes the viscosity of MDA-MB-231 cells to decrease during the adhesion process.which relates the standard viscosity and the cell adhesion characteristic through the impedance,and establishes the model of cell number-standard viscosity.