Alkaloid Components In Melodinus Yunnanensis And Their Network Pharmacology

Objective:Natural products have always been an important source of anti-tumor active substances,white solid wood monoterpene indole alkaloids are a class of alkaloids with significant antitumor activity,and vinblastine and reserpine drug molecules have been widely used in clinical practice.With the continuous development of science and technology,the pathogenesis of diseases has been continuously studied in depth,and network pharmacology has emerged.Network pharmacology is through the integration and computational analysis of data,which can systematically elucidate the interrelationship between drugs and compounds and many diseases,and explore the mechanism of action of drugs.Methods:This paper studies the chemical structure and antitumor activity of these alkaloids.To study the alkaloid composition and network pharmacology of Melodinus yunnanensis fruit of the oleander family.Silica gel,ODS,Sephadex LH-20 and other column chromatography techniques,thin layer chromatography,preparative liquid chromatography and other methods were used for separation and purification,and the chemical structure of compounds was identified according to their physical and chemical properties and spectroscopic data.Results: thirty-one alkaloids were isolated and elucidated as meloyunnanine D(1),scandine(2),scandine N-oxide(3),10-hydroxyscandine(4),tabersonine(5),11-methoxytabersonine(6),11-hydroxytabersonine(7),lochnericine(8),pachysiphine(9),11-hydroxylochnericine(10),19R-hydroxytabersonine(11),19R-a cetoxytabersonine(12),voaphylline(13),19S-vindolinine(14),19R-vindolinine(15),venalstonidine(16),17α-hydroxyvenalstonine(17),19R-methoxytubotaiwine(18),kopsiyunnanines F1(19),stemmadenine-N-oxide(20),stemmadenine(21),11-hydroxy-tabersonine(22),15β-hydroxyvincadifformine(23),(+)-vincadifformine(24),(-)-vincadifformine(25),Minovincinine(26),20-hydroxytabersonine(27),11-methoxy-15β-hydroxyvincadifformine(28),pleiocarpaman(29),11-octahydroxycarbazole(30),11-methoxycarboxylate(31)。Conclusion: the alkaloids in the fruits of Yunnan mountain orange were systematically recognized,and 31 compounds were isolated from the fruits of Yunnan mountain orange,in which compound 1-4 belonged to monoterpene quinoline type alkaloid,compound 5-19 penny 22-31 belonged to Baijiao type alkaloid,compound 20-21 belonged to strychnine type alkaloid.Scandine N-oxide(3),10-hydroxyscandine(4),11-hydroxytabersonine(7),lochnericine(8),pachysiphine(9),11-hydroxylochnericine(10),19R-hydroxytabersonine(11),19R-acetoxytabersonine(12)19S-vindolinine(14),19R-vindolinine(15),venalstonidine(16),17 α-hydroxyvenalstonine(17),19R-methoxytubot aiwine(18),kopsiyunnanines F1(19),stemmadenine-N-oxide(20),stemmadenine(21)were isolated from this plant for the first time.The chemical structures of the compounds were identified by silica gel,ODS,Sephadex LH-20 column chromatography,thin layer chromatography,preparative liquid chromatography and nuclear magnetic resonance.30 known monoterpene indole alkaloids were obtained,and a new monoterpene quinoline alkaloid was identified.31 monoterpene indole alkaloids were isolated,including 4 monoterpene quinoline type alkaloids,2 strychnine type alkaloids and 25 white pointed wood type alkaloids.Among them,1 is a new compound and belongs to monoterpene quinoline alkaloid,and the results show that the main type of monoterpene indole alkaloid in Yunnan mountain orange fruit is Baijian type.Isolated 31 monoterpene indole alkaloids were studied in the network pathology of liver cancer and leukemia.The study found that 16 compounds had potential active targets,among which DNMT1,HDAC1,STAT3,TP53 and other potential targets could play a role in the treatment of liver cancer by inhibiting HBx protein,which is necessary for viral replication,inhibiting somatic mutations,and regulating cell cycle.and potential active targets such as JAK2,JAK3,LCK,CXCR,ALK,MET,IGF1 R and other potential active targets may inhibit tumor cell proliferation and tumor cell migration by regulating ALK and glioma pathways,laying the foundation for finding active molecules.