Expression Of KIFC1 And Ki-67 In Breast Cancer And Its Relationship With Clinicopathological Features

Objective: To identify the diagnosed invasive ductal carcinoma of the breast,Non-special type(Invasive Ductal Carcinoma,IDC)of the patient with tumor tissue,adjacent tissue> 1cm from the tumor,Respectively examined the kinesin family members C1(Kinesin family member C1,KIFC 1)and the expression of Ki-67,To analyze whether there were differences in the expression of tumor tissue and pericancerous ductal epithelial cells;To explore whether the expression of the two is significantly different in different molecular types of breast cancer;The correlation between the expression and the clinicopathological characteristics of the patients were studied through data analysis;Validation of KIFC 1 expression in breast cancer tissues and normal tissues was performed using an online database.Methods:The expression of KIFC 1 and Ki-67 in 106 IDC tissues and adjacent ductal epithelial cells was detected by Immunohistochemistry(IHC).The statistical software SPSS26.0was used to analyze the correlation between the expression of KIFC 1 and Ki-67 in different molecular types of IDC tissues and the clinicopathological parameters of patients;the survival analysis was used to analyze the effect of its expression on prognosis;and the online database was used to query the expression of KIFC1 m RNA in breast cancer tissuesResults:(1)KIFC 1 and Ki-67 are not or poorly expressed in adjacent peripheral breast tissues,The expression rate of both in tumor cells was significantly higher than that of adjacent tissue;(2)The expression of KIFC 1 in IDC tissues is related to the histological grade of tumor,the maximum diameter of tumor,estrogen and progesterone(P <0.05),However,there is no correlation with the specific age of most patients,whether they are in the menopausal state,whether there is metastasis of lymph node,and the presence of vascular cancer thrombus(P>0.05);(3)Ki-67 was associated with histological grade of tumor,metastasis of lymph nodes and clinical stage(P <0.05),There was no relationship with the specific age of most patients,menopause,the diameter,ER,PR and HER-2 expression of the tumor(P> 0.05);(4)The results of Spearman’s correlation test show that,KIFC 1 was positively associated with Ki-67(r=0.4,P<0.0001);(5)The Kaplan-Meier survival analysis and Log-rank test results showed that,Compared to the patients with low KIFC 1 expression,Patients with high KIFC 1 expression had shorter disease-free relapse periods.(6)Univariate and multivariate results analysis in Cox proportional hazards regression model,KIFC 1 was an independent risk factor affecting the outcome of IDC patients(P <0.0001).(7)In the UALCAN database,KIFC1 m RNA was highly expressed in breast cancer tissues.In the analysis of clinicopathological data,the expression of KIFC 1 was found to be related to the tumor clinical stage,and the difference was also statistically significant in different molecular types,with P <0.05.Conclusion:1.KIFC 1 and Ki-67 are up-regulated in IDC tissues,which may participate in the occurrence of IDC;2.The elevated expression of KIFC 1 is related to pathological parameters such as higher grade,later clinical stage,larger tumor diameter and lymph node metastasis,suggesting that KIFC 1 may participate in the malignant progression in IDC;KIFC 1 is positively correlated with Ki-67 expression in 3..IDC,and KIFC 1 may participate in driving the proliferation of IDC and independent factors affecting the prognosis of breast cancer.