The Value Of Raman Spectroscopy-based Detection Of Circulating Tumor Cells In Breast Cancer Diagnosis

Background:Breast cancer is a common malignant tumor in women,with high incidence and metastatic tendency,and its metastatic sites are more frequent than other cancers.There is no convenient method to predict tumor metastasis,and circulating tumor cell(CTC)is a key factor for metastasis initiation.Recent studies have found that CTC can be used as a non-invasive detection indicator to warn tumor metastasis and determine disease progression.Raman spectroscopy is an optical analysis method to obtain information about the internal material of a sample,which has the advantages of simple sample preparation,fast detection speed and low cost.In this study,this technique was applied to CTC detection to detect CTC in a more advantageous way,and then guide the individualized treatment of breast cancer patients.Objective:Raman spectroscopy was used to detect CTC to provide a more effective method for identifying and characterizing CTC.To detect CTC in peripheral blood of breast cancer patients,analyze the correlation between CTC levels and clinicopathological indices of breast cancer,and evaluate the value of CTC in breast cancer diagnosis.Methods:1.Peripheral blood was collected from breast cancer patients according to the inclusion and exclusion criteria,and CTCs were isolated and enriched by the immunomagnetic bead positive screening method.a CTC classification model was established using Raman spectroscopy and multivariate statistical analysis.2.Immunofluorescence and real-time fluorescence quantitative PCR were selected to detect CTC and compared with Raman spectroscopy detection technique to analyze the advantages of Raman spectroscopy.3.The correlation between CTC levels and clinicopathological indices of breast cancer was analyzed based on the results of CTC testing.Results:1.There is a difference in the intensity of the characteristic peaks between CTC and leukocyte Raman spectra,and the difference indicates the different content of substances inside the two cells.A CTC classification model was developed with the existing training set and test set cells,and the accuracy of the model cell classification was up to 97.5%.2.The immunofluorescence method can identify different amounts of CTCs in different samples,and the real-time fluorescence quantitative PCR method can relatively quantify the CTC levels in peripheral blood.The Raman spectroscopy technique has the advantages of no labeling of the sample,fast detection and low cost compared with the traditional detection method of CTC.3.It was found that CTC was only present in the breast cancer group,and the level of CTC was significantly higher in the metastatic breast cancer group compared with the limited breast cancer group,and increased with the number of metastases.the number of CTC detection showed a significant positive correlation with tumor TNM stage,BI-RADS classification and serum CA153 level,and the difference was statistically significant.Conclusion:1.In this study,a classification model of CTC was established by Raman spectroscopy,which provides a more effective method for CTC detection.The method has the advantages of rich information,simple sample preparation,fast detection speed and low cost.2.The number of CTC detection in breast cancer patients was significantly correlated with the clinicopathological indices of breast cancer,and CTC detection has important application value in breast cancer diagnosis.