| Objective Combined with the application of Raman spectroscopy in biomedical, this study intends to application of Raman spectroscopy to identify group diff dyeing of circulating tumor cells and peripheral blood lymphocytes; Identification of Wright Giemsa staining of tumor cells and normal cells; Identification of liver cancer cell lines and peripheral blood lymphocytes; To identify and merge cirrhosis of the liver tissue and the liver cancer appraisal diaphragm foreign body.Methods Raman spectroscopy at the cellular level, this paper analyzes the Diff dyeing liquid samples by C TCs, CTCs lymphocytes and sample of membrane filter, dyestuff, slides and so on the Raman spectra of Wright Giemsa dyed samples of tumor cells and normal cells in Raman spectrum; Liver cancer cell lines and normal peripheral blood leukocytes of Raman spectra. At the organizational level analysis of 30 cases of liver cancer surgery in patients with liver cancer tissue and merge cirrhosis of the liver tissue of Raman spectra. In clinical case analyzes the foreign body in the mass of Raman spectra.Results Raman spectra by analyzing the sample, the result shows that: 1) At the cellular level, 532 nm wavelength laser dye by Diff CTCs, lymphocytes and the Raman spectra of dye in the 209~213 cm-1, 273~278 cm-1, 336~338 cm-1 have similar characteristics, such as peak; Under 785 nm wavelength laser by Dave staining of tumor cells, membrane and slides of Raman spectroscopy in 1200 ~ 2000 cm-1 area there are towering fluorescence peak, before the 1200 cm-1 area, 631~632 cm-1, 701~702 cm-1, 883~887 cm-1, 1106~1107 cm-1 of nasopharyngeal carcinoma cells and membrane filter has characteristics of Raman peaks, however the blank slides smooth curve. Under 532 nm wavelength laser by Wright Giemsa Staining of tumor cells and lymphocytes in 208~215 cm-1, 278~285 cm-1, 336~341 cm-1 peak near peak position have similar characteristics, such as shallow except in peak position due to the dyeing and dye 640~643 cm-1, 708~713 cm-1, 1500~1505 cm-1, 1620~1624 cm-1 Raman features peak higher, lower the remaining peak strength. White blood cells and cells around the base of Raman spectra curve flat, no significant characteristic peak; And liver cancer cells in peak position, 1449 cm-1, 1003 cm-1, 1666 cm-1 near visible characteristic peak. 2) At tissue level, from liver cancer tissue and merge cirrhosis of the liver tissue contrast figure of the Raman spectra can be seen: in peak position, 1155 cm-1, 1004 cm-1, 1510 cm-1 near merger cancer peak strength is higher than liver cirrhosis of the liver tissue; Near the peak 1642 cm-1 merger cirrhosis of the liver tissue of peak strength is lower than the liver cancer tissue; In 1377 cm-1 and 1586 cm-1 liver cancer tissue near significant characteristic peak and merger of cirrhosis of the liver tissue is not. 3) In clinical cases, foreign body from the diaphragm, Ann in fluorine and fluorouracil injection of Raman spectrum can be seen in the 412 cm-1, 1344 cm-1, 687 cm-1, 2876 cm-1, 1538 cm-1 and has obvious characteristic peak altitude of about the same.Conclusions By Raman spectroscopy analysis of Diff dyeing samples and Wright Giemsa samples Raman spectrum is visible through the dyeing samples after interference due to factors such as dye using Raman spectroscopy is unable to identify, by not dyed samples of liver cancer cell line and human peripheral blood lymphocytes of Raman spectrum analysis using Raman spectroscopy to identify tumor cells and lymphocyte is feasible. In 30 cases of liver carcinoma combined liver cirrhosis liver tissue and Raman spectra of contrast can be seen that using Raman spectroscopy to identify and merge the liver cancer of cirrhosis of the liver tissue is feasible. Through the Raman spectra of uncertainty about the nature of foreign body in clinical work, for our clinical work for foreign body provides a new means of identification. |
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