Association Of Rs4741506 Single Nucleotide Polymorphism Of SNAPC3 Gene With Ischemic Stroke And Its Chinese Medical Evidence

Objective:investigating the association of rs4741506 single nucleotide polymorphism in the small nuclear RNA activation complex polypeptide3 gene with ischaemic stroke（IS）,its TCM symptoms and related clinical and biochemical indicators.Methods:using case-control study,774 patients with stroke in the case group and 793 patients in the control group was included.In this study,genotyping tests were performed using Massarray SNP genotyping. Clinical index of IS were gathered from the system of hospital information.Analysis of the association between each genetic model and disease using unconditional logistic regression models;using general linear regression to find the connection of polymorphism with quantitative traits.PLINK software and SPSS 21.0 software were used for data analysis.Results:1.In the control group,the SNAPC3 gene rs4741506 was consistent with the HWE test(P_HWE=0.074).2.The genotype frequency distribution of SNAPC3 gene rs4741506locus was statistically different between the IS group and the controls（P=0.041）.The genotype frequency distribution of the SNAPC3 gene rs4741506 locus was statistically different between the IS phlegm evidence group（P=0.009）,blood-stasis group（P=0.045）and the controls;The genotype frequency distribution of the other TCM evidence group of IS was not statistically different compared to the control group（all P>0.05）.3.No statistically significant association between the rs4741506polymorphism of the SNAPC3 gene and the risk of IS in any genetic model（all P>0.05）.Stratified analysis by gender:the difference between the SNAPC3 gene rs4741506 polymorphism and the risk of developing IS in female patients was statistically significant(dominant model:P_adj=0.038).Across genetic models,the association of the rs4741506polymorphism of the SNAPC3 gene with IS in male patients was not statistically significant（all P>0.05）.4.The rs4741506 pollymorphism in the SNAPC3 gene was strongly correlated with the incidence of occurrence of IS wind evidence(dominant model:P_adj=0.029)and phlegm evidence(dominant model:P_adj=0.011).The association analysis with the risk of developing IS fire-heat and qi-deficiency evidence was not statistically significant（P>0.05in all genetic models）.5.rs4741506 of the SNAPC3 locus was significantly associated with the phlegm score in IS patients(recessive model:P_adj=0.017).No statistically significant associations were found with the Wind,Fire-Heat,Blood-Stasis and Qi-Deficiency syndrome scores in IS patients（P>0.050 in all genetic models）.6.Association analysis of the rs4741506 polymorphism of the SNAPC3 gene with clinical indicators related to IS patients（i）Analysis based on the total sample.The SNAPC3 rs4741506 polymorphism was associated with FPG(additive model:P_adj=0.008;dominant model:P_adj=0.007),APO-A(dominant model:P_adj=0.013),PLT(additive model:P_adj=0.011;dominant model:P_adj=0.019),and TT(dominant model:P_adj=0.040)in IS patients.model:P_adj=0.040),and FIB(dominant model:P_adj=0.046)were statistically significant.（ii）Analysis according to TCM evidence grouping.SNAPC3 gene rs4741506 polymorphism was associated with serum APO-B(recessive model:P_adj=0.037)and DBP(recessive model:P_adj=0.028),APO-A(additive model:P_adj=0.023),dominant model:P_adj=0.016)and PLT(additive model.P_adj=0.021,dominant model:P_adj=0.039)and TT(dominant model:P_adj=0.048)levels were statistically significant.Conclusions:1.The rs4741506 polymorphism of the SNAPC3 gene may be associated with the risk of developing wind and phlegm in ischaemic stroke.2.rs4741506 polymorphism of SNAPC3 gene may be associated with Apo B levels in patients with ischaemic stroke.3.rs4741506 polymorphism of SNAPC3 gene may be associated with diastolic blood pressure,apolipoprotein A,platelet and prothrombin time levels in patients with ischaemic stroke with phlegm.