Scutellaria Baicalensis Polysacchdride Alleviates The Intestinal Inflammation Of Ulcerative Colitis Mice By Regulating JAK2/STAT3 Pathway And IL-23/IL-17 Inflammatory Axis

ObjectiveTo analyze the effects of scutellaria baicalensis polysaccharide(SBP)on intestinal inflammation in mice with ulcerative colitis(UC)and its mechanism.MethodsSixty C57BL/6 mice were used for related experiments,and randomly divided into blank group,model group,mesalazine group(100mg/kg),low,medium,and high-dose SBP groups(50,100,and 200mg/kg),according to body mass.Each group consisted of 10 animals.Except for the blank group,the mice in the other groups drank 3%DSS aqueous solution freely for 7 consecutive days to establish the UC model,at the beginning of modeling,the drug was given intragastric administration according to the above groups.The blank group and the model group were given the same amount of normal saline(20ml/Kg),once a day,for 10 days.After completion,disease activity index score(DAI)was compared in each group to evaluate the therapeutic effect of SBP.The serum levels of IL-6,IL-17 and IL-23 were detected by enzyme-linked immunosorbent assay(ELISA);HE staining was used to observe the colonic mucosal injury of mice and the colonic mucosal injury score(TDI)was recorded;The expression levels of JAK2,STAT3 and their phosphorylated proteins in colon tissues were detected by Western blot and immunohistochemistry.Results1.Compared with the model group,the DAI scores of the mesalazine group,the high and medium dose of SBP groups were decreased(P>0.05);Compared with mesalazine group,the DAI score of low dose SBP group increased,and the differences were statistically significant(P<0.05),there was no significant difference in DAI scores between the high,medium dose groups and the mesalazine group(P>0.05).2.Compared with the model group,the TDI scores of the mesalazine group and the high-dose SBP group decreased(P<0.05),and the colonic mucosal injury was mild.Compared with mesalazine group,the TDI scores of medium and low dose SBP groups increased,and the differences were statistically significant(P<0.05).There was no significant difference in TDI score between the high dose of SBP group and mesalazine group(P>0.05).3.ELISA results showed that compared with the model group,the levels of IL-6,IL-17 and IL-23 in the mesalazine group and the high,medium and low dose SBP groups were decreased.Compared with the mesalazine group,the levels of IL-6,IL-17 and IL-23 in the high,medium and low dose SBP group were increased,the contents of IL-6,IL-17 and IL-23 decreased with the increase of the dose of SBP,and the differences were statistically significant(P<0.05).4.WB and immunohistochemistry results showed that compared with the normal group,the p-JAK2/JAK2 and p-STAT3/STAT3 ratios were increased in the model group,mesalazine group,and high,medium and low dose SBP groups(P<0.05),Compared with the model group,the ratio of p-JAK2/JAK2 and p-STAT3/STAT3 in mesalazine group,high,medium,low-dose scutellaria baicalensis polysaccharide groups decreased,and the differences were statistically significant(P<0.05).Conclusions1.SBP can reduce the inflammation of UC,which may play a role through IL-23/IL-17 inflammatory axis.2.SBP may improve intestinal inflammation in UC mice through JAK2/STAT3 pathway.