Research On The Role Of BDNF And Its Genetic Polymorphism(Val66Met) In Patients With Alcohol Dependence Syndrome

Objective:To investigate the role of BDNF and its gene polymorphism rs6265 in patients with alcohol dependence syndrome.Methods:Fifty-nine male patients who were admitted to the Psychiatric Hospital of Yunnan Provinceduring the period from September 2019 to November 2019 and meet the diagnostic criteria for alcohol-induced mental and behavioral disorder-dependent syndrome caused by ICD-10,and matched with Thirty-seven male healthy controls group,rs6265 loci of BDNF gene polymorphism was tested and ELISA for proBDNF and mBDNF concentrations in plasma,and analyzing their relationship with alcohol dependence syndrome.Results:1.Hardy-Weinberg equilibrium test:The control group and the case group were respectively tested for the genotype distribution of the rs6265 locus and tested for goodness-of-fit ?2 test,the difference between the observed value and the theoretical value was not statistically significant(Control group:?2=0.248,P=0.619;Case group:?2=1.150,P=0.284),in line with Hardy-Weinberg equilibrium,indicating that the two groups of subjects are from a large group,The samples are somewhat representative.2.There was no significant difference in the genotype(MetMet,ValMet,ValVal)allele A/G at the rs6265 locus between the case group and the control group.3.Compared with the healthy control group,The plasma proBDNF expression level of alcohol-dependent patients was increased,and the difference was statistically significant.4.Compared with the healthy control group,the plasma mBDNF expression level of alcohol-dependent patients was decreased,and the difference was statistically significant.5.The univariate analysis of variance showedthat there was no statistical difference in plasma proBDNF and mBDNF levels between different genotypes of rs6265 in the case group,but there was a trend of difference.The plasma proBDNF level in GG genotype patients was lower than that in GA and AA genotype patients,while that in GA genotype patients was lower than that in AA genotype patients.The plasma mBDNF level of GG genotype patients was higher than that of AA genotype patients.6.plasma proBDNF concentration in the case group was a positive correlation with the average daily alcohol consumption in recent month,r=0.344,P=0.008,the plasma mBDNF concentration in the case group was negatively correlated with the average daily alcohol consumption in recent month,r=-0.361,P=0.005,the plasma of the case group The concentration of proBDNF was positively correlated with the age of drinking,r=0.317,P=0.014,the plasma mBDNF concentration of the case group was negatively correlated with the age of drinking,r=-0.427,P=0.001.Conclusion:There is an imbalance between proBDNF and mBDNF in patients with alcohol dependence syndrome,which may be a biological mechanism for the development of alcohol dependence syndrome,the BDNF signal transduction pathway can be studied as a stable regulatory pathway for alcohol addiction.The BDNF gene polymorphism rs6265 has no significant effect on the levels of proBDNF and mBDNF.Gene polymorphisms were not related to the onset of alcohol dependence syndrome in the Han population.There were many factors affecting the levels of proBDNF and mBDNF.Plasma proBDNF levels were positively correlated with drinking yearsandthe average daily alcohol consumption in recent months.Plasma mBDNF The level was negativelycorrelated withdrinking yearsandthe average daily alcohol consumption in recent months.