| Object:To investigate the relationship between intestinal oxalate bacteria and nanobacteria in the formation of kidney stones in Wistar rats.Methods:180 healthy male Wistar rats were randomly divided into experimental group,control group and interference group,and treated with different treatments:(1)Experimental group(n=60):1.2 ml of nanobacteria suspension+2 ml of 0.9%sodium chloride solution was injected through tail vein for intragastric administration;(2)Control group:2 ml of 0.9%sodium chloride solution with the same amount of normal saline was injected into tail vein.(3)In the interference group,the same amount of nanobacteria suspension+2 ml tetracycline solution was injected into tail vein.The formation of kidney stones in rats was detected at different time periods,abdominal aortic blood was collected to detect inflammation-related indexes,the localization of inflammatory cells in kidney was observed by immunofluorescence technique,and fresh feces in ileocecum were collected.The gene fragment of Oxacillus formate in intestinal tract was detected by real-time quantitative PCR technique,and the dynamic changes of kidney inflammation and Oxacillus formate in intestinal tract during the formation of experimental kidney calculi induced by nano-bacteria were studied dynamically.Results:(1)Microscopic observation showed that the kidneys of rats in the experimental group formed crystals from the 4th week,and the crystals were the most.During the same period,no crystals were formed in the interference group and the control group.At the 6th week,a large number of crystals formed in the kidneys of the experimental group,which were connected into pieces,and the kidneys in the interference group appeared.There was a small amount of crystals,and no crystal formation was found in the control group;the difference in the positive rate of crystals between the experimental group and the control group was statistically significant(P<0.01),and the difference in the positive rate of crystals between the experimental group and the interference group was statistically significant(P<0.05).(2)Flow cytometry showed that CD3~+T lymphocytes were well expressed in blood samples of rats in each group,and there was no significant difference among the three groups.The data of the first 4 weeks showed that the differences in the expression levels and changes of CD4~+T lymphocytes and CD8~+T lymphocytes in the blood of the three groups of rats were small,and the differences among the three groups were not statistically significant(P>0.05).The expression level of CD4~+T lymphocytes in the blood of the group was higher,the expression level of CD8~+T lymphocytes was lower,and the ratio of CD4~+/CD8~+in the blood of the experimental group was higher.The expression level of CD4~+T lymphocytes in the blood of the rats in the control group decreased significantly,and the CD8~+T lymphocytes gradually increased.The CD4~+/CD8~+ratio of the rats in the control group decreased significantly compared with the other two groups.The expression level of CD4~+T lymphocytes in the blood of the rats in the interference group decreased,and the expression of CD8~+T lymphocytes increased.The ratio of CD4~+/CD8~+in the blood of the rats in the interference group showed a downward trend.,CD4~+/CD8~+ratio difference was statistically significant(P<0.05).At the 10th week,the CD4~+T lymphocytes,CD8~+T lymphocytes and CD4~+/CD8~+ratio in the blood of the experimental group,the control group and the interference group were not significantly different(P>0.05).(3)Immunofluorescence results showed that:a large number of CD4~+T lymphocytes were obviously stained in the glomerulus of renal cells in the experimental group,while the control group had the least red staining,that is,the least CD4~+T lymphocytes,and the interference group was between the two.CD8~+T lymphocytes were also infiltrated in the kidneys of the three groups of rats.In the control group,a large number of CD8~+T lymphocytes were infiltrated in the glomeruli in green.The number of CD8~+T lymphocytes infiltrated in the interference group was moderate.(4)Fluorescence quantitative PCR data showed that:from the first week to the fourth week,the relative expression of Oxc m RNA in the experimental group,the control group and the intervention group had little difference,and there was no statistical significance(P>0.05).In the ninth week,the relative m RNA expression of Oxcobacillus formate formate-producing Oxc in the experimental group decreased significantly compared with the control group,and the difference was statistically significant(P<0.01).Obviously,the difference was statistically significant(P<0.01).Compared with the rats in the interference group,the difference in the relative expression of Oxc m RNA was small,and there was no statistical significance(P>0.05).The 10th week showed that the relative expression levels of Oxc m RNA in the three groups of rats tended to be the same,and the difference was not statistically significant(P>0.05).Conclusion:Nanobacteria can cause inflammation in the whole body and kidney of experimental rats.During the formation of experimental kidney calculi in rats induced by nanobacteria,the number of oxalic acid-producing bacilli decreased,and the number of oxalic acid-producing bacilli increased after anti-nanobacteria treatment. |
PDF Full Text Request