Study The Basis Of Anti-inflammatory Substances Of Fibraurea Recisa Pierre.Based On The Components Cross-compatibility

The dried rattan stems of Fibraurea recisa Pierre.belongs to Menispermaceae,which is mainly distributed in Yunnan,Guangxi,Guangdong and Vietnam,Cambodia and Myanmar.Its taste is bitter,cold to the heart,liver meridian,has the effect of clearing away heat and detoxifying,purging fire and defecating,and it can prevent and cure various infectious diseases,such as gynecological inflammation,surgical infection,dysentery,enteritis,respiratory tract infection,ophthalmic conjunctivitis and so on.The main chemical components of F.recisa Pierre.are alkaloids,lactones and so on.In recent years,due to the increasing lack of resources of F.recisa Pierre.,the research on its chemical components is at a standstill.The pharmacological effect focuses on the anti-inflammatory and bacteriostatic effects of F.recisa Pierre.,while there is a lack of corresponding in-depth research on other active components.Therefore,based on the component cross compatibility method,this thesis studies the COX-2 inhibitory activity of F.recisa Pierre.,studies its effective components on the basis of clarifying its anti-inflammatory activity,and forms effective active components better than single components,in order to provide some ideas and methods for the basic research of compatibility of traditional Chinese medicine.In our experiment,the 70%ethanol extract of F.recisa Pierre.was chromatographed on silica gel column,and 60 components were obtained after TLC detection.30 components were sampled at intervals to study the inhibition of COX-2 activity.The inhibition rate was calculated according to the measured RFU,and then the IC₅₀value was calculated to judge the inhibition effect of components on COX-2.The results showed that 21 components of F.recisa Pierre.had good inhibition effect on COX-2,it can be considered to be researched in order to find better bioactive compounds in the future.After cross compatibility of components and comparison with single component,it is found that the activity of each component is not as good as that of a single component.Only component 3+57,component5+55 and component 11+49 can enhance the activity of a single component,so it is considered not to conduct further research.In our research,the ethanol extract of F.recisa Pierre.was systematically separated and purified.16 compounds were isolated by silica gel column chromatography,D101macroporous resin column chromatography,ODS column chromatography,thin layer preparation chromatography and Pr-HPLC.The structures of the isolated compounds were elucidated on the basis of comprehensive analysis of UV,IR,HR-MS,1D and 2D NMR and its physical and chemical properties.Isolated compounds were identified as corydeamine（1）,syringalsucammide（2）,palmatine（3）,jatrorrhizine（4）,pseudocolumbamine（5）,calonysterone（6）,stachysterone A（7）,stachysterone B（8）,rubrosterone（9）,14α,15α-epoxy-14,15-dihydrostachysterone B（10）,fibleucin（11）,β-sitosterol（12）,palmitic acid（13）,trigonelline（14）,isovanillin（15）,daucosterol（16）,stigmasterol（17）.Among them,compound 1 and compound 2 are new compounds,compounds 6-10 and 14 are isolated from F.for the first time,and some compounds are identified by UPLC（ultra-perfoemance liquid chromatography）,which laid a foundation for the further study of F.recisa Pierre.In our study,an integrated characterization strategy by combing mass defect filtering-based structure classification and diagnostic fragment-ion-based extension was proposed for rapid profiling of chemical compositions in F.recisa Pierre.via ultra-perfoemance liquid chromatography/quadrupole time-of-flight mass spectrometry（UPLC-Q-TOF-MS）.The total ion chromatogram of the ethanol extract of F.recisa Pierre.were obtained by electrospray ionization（ESI）in positive ion modes,and compounds of the genus F.based on the related literature was analyzed by combing UNIFI software with manual work,and only 20 chemical components were identified,due to the existence of a variety of isomers in F.recisa Pierre.,it was very difficult to analyze by mass spectrometry According to the accurate mass information of excimer ions and fragment ions,the molecular composition of the compounds was preliminarily speculated,and then the cracking law of the compounds was referred according to the references.In our research,the molecular docking of COX-2 protein of 15 compounds isolated from F.recisa Pierre.was carried out by using Auto Dock Vina.It was found that the docking results were less than-5 kcal/mol,indicating that these 15 compounds had good binding activity,which can be further studied in the future.In our study,the COX-2 inhibitory activity of the chemical constituents isolated from F.recisa Pierre.was studied.The results showed that there was no obvious regularity in the inhibition of COX-2.