Expression And Clinical Significance Of IL-33,MVD And LVD In Carcinoma And Paraneoplastic Tissues Of Hepatocellular Carcinoma

Background and objective: Interleukin-33(IL-33)is a dual-role cytokine that belongs to the IL-1 cytokine family.It also acts as a chromatin-associated factor in the nucleus,regulating gene transcription by exhibiting the properties of a transcriptional repressor,and plays an important role in the development of inflammatory diseases and many malignancies.However,the clinical significance of IL-33 in the development and prognosis of hepatocellular carcinoma(HCC)is unclear.In this paper,we investigated the different expression of IL-33,lymphatic vessel density(LVD)and microvessel density(MVD)in HCC cancer tissues and paraneoplastic tissues,and analyzed and studied the correlation and clinical significance of IL-33,MVD and LVD with clinical and pathological parameters of patients.The correlation and clinical significance of IL-33,MVD,LVD and HCC were analyzed and studied in relation to the clinical and pathological parameters of patients.Methods: A total of 62 patients who underwent surgical resection for HCC in the Hepatobiliary Surgery Department of Qingdao Municipal Hospital from 2014 to 2016 were selected for this study.Inclusion and exclusion criteria for sample selection were established,and the follow-up clinical data of patients eligible for inclusion were retrospectively analyzed.The expression of IL-33 protein,MVD and LVD in the patients’ cancer and paracancer tissues was measured by immunohistochemical staining(SP method),and the possible correlation between the the clinicopathological parameters and three of the enrolled HCC patients was analysed.The patients’ disease progression status was followed up and the correlation with the progression-free survival(PFS)of HCC patients was analysed.All statistical analyses were performed using SPSS 24.0 for statistical summary analysis.Data were recorded in a four-cell table format,with χ2 tests used for comparisons between groups and log-rank tests for the correlation between IL-33 expression and progression-free survival(PFS)in HCC patients.One-way and multi-way Cox regression analyses were used to statistically correlate IL-33,MVD,LVD and clinicopathological data with PFS in HCC patients.p-value < 0.05 was considered a statistically significant difference.The study was approved by the Medical Ethics Committee of Qingdao Municipal Hospital.Results:Combining immunohistochemical staining results and statistical analysis data,the expression of IL-33 was higher in HCC paraneoplastic tissues than in cancer tissues,The difference was statistically significant(P < 0.01).The expression of MVD in HCC was higher than that in adjacent tissues(P < 0.01),and there was no significant difference in LVD expression(P>0.05).The intensity of IL-33 expression was related to MVD expression,tumor T-stage(P<0.05)and lymph node metastasis(P<0.01),but not significantly correlated with age,sex,history of alcohol consumption,hepatitis and cirrhotic background(P>0.05).lymph node metastasis status,IL-33,clinical T stage MVD,and tumor pathological histological grade obtained by univariate and multifactorial Cox regression analysis were associated with PFS.Conclusions: High expression of IL-33 in paraneoplastic tissues of HCC patients is associated with poor prognosis of patients.High expression of IL-33,high expression of MVD,lymph node metastasis,advanced clinical T stage and poor histological grading of tumor pathology are risk factors for poor prognosis of patients with PFS,except for histological grading of tumor pathology,the other four are independent risk factors for the duration of PFS.IL-33 may as prognostic IL-33 may provide new directions and strategies for the diagnosis and treatment of HCC as a predictive factor.