Molecular Correlation Identification Of EPRS Gene In Myocardial Noncompaction

Objective:To analyze and screen the key genes in myocardial non-compaction by bioinformatics.Methods:In this study,By downloading and extracting the m RNA expression matrix of the GSE178582 microarray data set,the differentially expressed genes of the normal control group and the myocardial non-compaction group in the data set were compared,and the function and pathway enrichment analysis was performed by GSEA.The STRING database was used to establish the protein interaction network,which was visualized by Cytoscape software,and the key genes were further screened by the Cyto Hubba plug-in.Finally,the key genes were verified in the GSE103673 dataset.Results:After analysis by R language,264 differential genes were screened,of which 211 genes were significantly up-regulated,and 53 genes were significantly decreased in expression.GSEA enrichment analysis showed that the GO enrichment in the myocardial non-compaction group was concentrated,and the biological process was mainly concentrated in iron ion translocation.Membrane transport,apoptotic intercellular space,activation of adenylate cyclase activity,metabolic process of glutathione derivatives and other processes;molecular functions mainly focus on transition metal ion transmembrane transport activity,glutathione transferase activity and phosphatidylinositol phosphatase activity;cellular components are mainly concentrated in keratin fibers,Calyx-like synaptic terminals,and Cop II vesicle coatings.The KEGG pathway is enriched in drug metabolism cytology and Isopropyl acid metabolism by cytochrome P450.After analyzing and verifying the key genes through protein interaction,the EPRS gene was screened as the key gene in myocardial non-compaction.Conclusion:EPRS has significant differences in the expression of myocardial noncompaction,and is expected to become a biomarker and therapeutic target for myocardial noncompaction.