Study On Weight Loss Effect Of ProbioticsStudy On Weight Loss Effect Of Lactobacillus Paracasei K56,Bifidobacterium CP-9 And Lactobacillus Reuteri

Objective:To explore the weight loss effect of animal bifidobacterium LP1 group(Animal Bifidobacterium,CP-9),Lactobacillus Royale LP2 group(L.reuteri)and Lactobacillus parasitum LPC1 group(Paracetamolactic acid bacteria,K56)on obese mice induced by high fat diet.Methods:Six-week-old male C57BL/6J mice were fed with high-fat diet for 8 weeks,and 96 obese mice were selected and divided into 8 groups,which were fed with continuous high-fat diet and intervened with corresponding probiotics for 10 weeks.They were LPC1 group(K56,107 cfu/d,108cfu/d,109cfu/d,1010cfu/d,1011cfu/d),LP1 group(CP-9,109cfu/d)and Lactobacillus Royeri group(L.reuteri,109 cfuiamd).In addition,12 normal mice of the same age were randomly selected as blank control.After the intervention,the effect of probiotic intervention on obese mice was evaluated by combining body weight,body composition,nuclear magnetic resonance,fat weight,blood glucose,blood lipids,expression of FAS gene and PPAR y gene protein in liver,intestinal flora and short-chain fatty acids to explore the possible mechanism.The results were expressed by(x ±s)or median(quartile)(Median(IQR),and the comparison between groups was carried out by analysis of variance or nonparametric test.The probability level of hypothesis test is α=0.05.Results:(1)At the end of the intervention,the body weight of the intervention group was significantly lower than that of the model control group,and the weight loss was mainly reflected in the total fat weight.Including subcutaneous fat and abdominal fat(perirenal fat,peri-epididymal fat,mesenteric fat)decreased significantly,but the weight of lean tissue did not change significantly.(2)The intervention of three kinds of probiotics could effectively reduce the concentration of plasma total triglyceride(Triglyceride,TG)and low density lipoprotein cholesterol(Low-Density Lipoprotein Cholesterol,LDL-C)in obese mice,but the concentration of total cholesterol(Total Cholesterol,TC)in obese mice did not decrease significantly.(3)The intervention of three kinds of probiotics could reduce the concentration of blood glucose,reduce the level of glycosylated hemoglobin,improve the ability of blood glucose regulation,slow down the increase of insulin level,and restore islet β-cell function to a certain extent.(4)Probiotic intervention can improve the abundance,evenness and diversity of intestinal colonies.Body weight and blood lipids are related to the composition and metabolism of specific bacteria.Probiotics play a role by affecting the abundance of specific bacteria.(5)The intervention of three probiotics can regulate the proportion of fatty acids in the feces of obese mice induced by high fat,alleviate the decrease of the level of total short-chain fatty acids to some extent,and have more definite evidence for the increase of the contents of valeric acid and isovaleric acid in feces.Conclusion:Probiotic intervention can effectively slow down the weight gain of mice induced by high fat diet,which may affect the levels of plasma cholesterol and triglyceride by regulating the activities of FAS gene and PPAR y gene in liver,reduce fat accumulation in obese mice,and affect the composition and metabolism of intestinal flora.Probiotic intervention can effectively improve glucose and lipid metabolism in obese mice and control blood sugar.