A Study Of Constitutional Science Of Chinese Medicine Distribution And Serum Lipidomics Characteristics Of Ankylosing Spondylitis

Objective:To survey and analyze constitutional science of Chinese Medicine theory through ankylosing spondylitis patients from 5 hospitals in Zhejiang province and Chinese medicine hospital of Jiangxi province,and probe the regularities of distribution of constitutional science of Chinese Medicine of those ankylosing spondylitis patients.And analyze the difference of serum lipidomics between ankylosing spondylitis patients and normal person by multi-dimensional mass spectrometer-based shotgun lipidomics(MDMS-SL)and Gas Chromatography-Mass Spectrometer(GC-MS),and then study the characteristics of lipid molecule of ankylosing spondylitis patients,try to elaborate the Pathogenesis from the view of abnormal lipid metabolism.Methods:1:Using the data obtained from 122 ankylosing spondylitis patients from ankylosing spondylitis patients from Sep.2015 to Jun.2016,using Standardized TCM constitution measuring standard to evaluate scores of all kinds of constitutions,judging every patients’ constitution type.2.35 male AS patients(12 phlegm-wetness type,12 Yang deficiency type,11 normal type)and 35 normal counterparts were recruited in the study.MDMS-SL was performed to analyze the lipids extracted with chloroform/methanol solvent system from their sera.3.In order to further understand the underlying mechanisms for the decreased PE,quantify ethanolamine and free cholesterol in the sera were quantified using GC-MS.After acquired the corresponding spectrum,their peaks were identified,normalized to the internal standard in MSD work station and then identify and repeated analyze the peaks,integral in MSD work station,and then calculated their mass levels.Results:1.Among the 35 AS patient,8 biased constitutions occupied 85.2%,and among the 8 constitutions,Yang deficiency types and phlegm-wetness types take 27.05%and 19.67%;different genders,ages,living environment and family are related to AS pathogenesis;2.Lipid extracts from the sera of 35 AS patients(12 phlegm-wetness types,12 Yang deficiency types and 11 normal type)and 35 normal person were analyzed in the research.There was no difference of lipid profiles between the three constitutions of AS patients,including Car,PI,PE,LPE,SM,PC,LPC and CER.However,there were some significant differences between AS patients and the normal in PE,LPE,LPC,and SM.Compared to the normal,PE,LPE and SM in AS patients were obviously reduced,and most of LPC species were also decreased.3.The contents of ethanolamine and free cholesterol in sera of 35 AS patients and 35 were quantified using GC-MS.There were no statistics differences of them between AS patents and the normal.Conclusion:1.By analyzing AS Chinese constitution we have known,85.2%AS patients are biased constitution,and Yang deficiency types took the largest part,phlegm-wetness types followed;Different genders,ages,living environment,and family are closely related to AS pathogenesis.2.Lipidomics study revealed that the synthetic pathway of PE in AS patients were aberrant,resulting in the significant reduction of PE species.The decrease of PE may be associated with AS pathogenesis.Besides,the results also showed that oxidative stress was elevated in AS patient.On the other hand,due to the limited number of samples,this study failed to give the difference of lipid metabolism in patients with different constitution AS,and more samples were needed.