Regulation Of Tuberostemonine On Liver Giucolipid Metabolism

With the emergence of modern intensive feeding mode,the incidence of animal fatty liver is rapidly increasing,which a nutritional metabolic disease,and mostly occurs in laying hens,dairy cows and laying ducks who closely related to people’s life,which greatly affects the development of animal husbandry.Available studies have shown the positive effects of plant extracts in the prevention and treatment of fatty liver in animals.However,plant extracts are diverse and widely available,there are still relatively few applications in the prevention and treatment of fatty liver.Therefore,there are more cost-effective plant extracts with less toxic side effects waiting to be discovered for application.Our subject group has previously discovered through large-scale screening using OA/PA-induced lipid accumulation model in Hep G2 cells that tuberostemonine,a bioactive substance extracted from the traditional Chinese medicine Stemona tuberosa Lour,has excellent lipid-lowering effects and is inexpensive.In this thesis,the effects of tuberostemonine on hepatocyte glycolipid metabolism and the alleviation of fatty liver were investigated at the cellular and animal in vivo levels,to provide a theoretical reference for its further development and application.Cellular experiments were conducted to investigate the effect of tuberostemonine on the glycolipid metabolism of OA/PA-treated hepatocytes using Hep G2 cells as a model.The results showed that the addition of 50 μM tuberostemonine treatment significantly decreased hepatocyte triglyceride(TG)and total cholesterol(TC)contents;reduced the production of reactive oxygen species(ROS)and lipid peroxide product malondialdehyde(MDA);increased glutathione(GSH)and catalase(CAT)activities;increased ATP production and the amount of mitochondrial membrane potential;and promoted hepatocyte glycogen storage capacity and glucose uptake capacity.Animal experiments were conducted using C57BL/6J mice as a model to investigate the regulatory effects of tuberostemonine on hepatic glucolipid metabolism in mice.Forty seven-week-old male C57BL/6J mice were randomly divided into 4 groups of 10 each:(1)normal standard feed diet group(ND),(2)high fat feed diet group(HFD),(3)high fat feed diet group with intraperitoneal injection of low dose tuberostemonine(15 mg/kg)(HFD-L),(4)high fat feed diet group with intraperitoneal injection of high dose tuberostemonine(30 mg/kg)(HFD-H),conducted formal in vivo experiments for 11 weeks.The results showed that tuberostemonine treatment significantly inhibited body weight and adipose tissue weight gain of mice induced by high-fat diet;reduced liver TG and TC content in mice;alleviated hepatic steatosis and liver injury;improved insulin resistance and glucose intolerance in mice;regulated energy metabolism in mice.The present study confirmed that tuberostemonine has an important regulatory effect on hepatic glucose and lipid metabolism and can alleviate hepatic steatosis and liver injury,providing a theoretical reference for the development of new feed additives and related drugs,offering new ideas for the prevention and treatment of fatty liver in livestock and poultry,and expanding the application of natural products of plant origin in livestock and poultry breeding industry.