| Agropyron cristatum(Linn.)Gaertn.is a perennial herbaceous plant in Herchi.Inner Mongolia has the largest distribution,high distribution density,strong stress resistance,and high feed value,ecological value and germplasm resource protection and utilization value.The research made by the predecessors mainly focused on the development and utilization of resources.The internal relationship between the morphological traits of ice grass has not been systematically counted.Cognition.Therefore,this article uses the phenotype character and SSR molecular labeling to identify the genetic diversity of 28 copies of Agropyron cristatum germplasm resources to reveal reveal the correlation between the external phenotype of Agropyron cristatum and its intrinsic genes,and provide the evaluation and selection of Agropyron cristatum.in accordance with.Below are key research findings:1.The 22 main phenotypic traits of Agropyron cristatum differ significantly,with the coefficient of variation of the first segment length of shaft,leaf number,tiller number and external length,which can be used as indicators for resource identification,evaluation and quality breeding.The changes between the quality and quantitative genetic diversity index of Agropyron cristatum species resources are 0.96 ~ 1.70 and 1.09 ~ 2.12,respectively.The main component analysis selected the accumulated contribution rate of the first 7 main components such as the number of blades,the density of the ears,and the ears of the spikes reached 75.415%.The system clustering study of Agropyron cristatum shows that these species can be divided into 4 major branches.2.Filter 12 polymorphism and higher primers.12 A total of 107 equivalent genes were detected on the SSR primer,with an average of 8,the average Ne was 4.79,the average Ho was 0.29,the average He was 0.66,the average UHe was 0.68,and the average I was 1.51,indicating that 28 copies of Agropyron cristatum materials materials were high genetic diversity is high.3.The FST range of the ice grass group is 0.01 ~ 0.07,there are 9 of Fst < 0.05,and3 of 0.05 < Fst <0.15,which shows that the degree of differentiation of each point of Agropyron cristatum is different.The gene flow is 3.46 ~ 29.30,indicating that genetic exchanges between ice grass groups are frequent.Molecular variance analysis shows that37% of the genetic differences are from the group,and 63% of the hems of Agropyron cristatum group comes from the group.It can be divided into four categories by UPGMA at a genetic coefficient of about 0.78.4.The results of the group’s genetic structure analysis show that when K = 4,ΔK reaches the maximum value,and the 4 sub-groups of Agropyron cristatum have obvious differences.The individual "8","11" and "5" have high independence There are genes between other individuals crossing and penetrating.5.Analysis of the correlation analysis of the Agropyron cristatum phenotype and the SSR molecular marker detected that 16 characteristics are associated with 10 SSR marks,the 5 characteristics of the base stem thick,the first section of the ear shaft,and the number of small ears are all with 2.The above sites are significantly related,indicating that some traits are jointly regulated by multiple genes;J-30,Xgwm217,and 7 signs including Xgwm249 are significantly related to multiple characteristics,reflecting genetic multi-effects. |
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