| Wheat products have a wide range of edible value,but high-quality germplasm resources are still scarce at present,and the research on the synthesis mechanism of its main nutritional components has not yet become a system.On the basis previous studies,this study continued to study the location of quality traits,and at the same time carried out the exploration of comprehensive analysis of proteomics and metabolomics of grain filling.The following results were obtained:(1)202 GZ-RILs and HMW-GS of their parents were identified by SDS-PAGE,and there were 6 subunit types at Glu-1 locus,and 23 subunit combinations were obtained,among which the high-quality subunit combinations 1,7+8 and 5+10 appeared most frequently.(2)99 loci of GZ population were mapped to form 21 linkage groups,with a total length of 5,039.12 c M and an average distance of 4.15 c M;between markers;Based on the constructed 16K-GZ wheat molecular marker genetic map,the QTL mapping of eight main quality traits of wheat in four environments was analyzed by using complete interval mapping method.A total of 97 additive QTLs were detected,distributed on 21 chromosomes of wheat,and 47 QTLs were major QTLs with genetic contribution rate exceeding 10%.QWgc-5D-1,which controls wet gluten content,is located between 5D＿438049329-5D＿311466197 and 6D＿211432960-6854074 on 5D chromosome,and can explain 22.0745% phenotypic variation respectively.(3)5,405 protein with significant differences in expression were identified by the comparative proteomics of Lable-free,and DEPs mainly concentrated in the biological pathways such as glycolysis,TCA and alcohol fermentation,post-translation modification,energy generation and transformation,amino acid transport and metabolism,sucrose metabolism and starch biosynthesis,storage protein metabolism and secondary metabolites biosynthesis,transportation and catabolism.606 compounds were identified by LC-MS nontarget metabolomics.Qualitative and quantitative analysis showed that the main metabolic activity during grain filling was the synthesis of small molecular substances,including sucrose,amino acids,lipids,etc.,and lipid substances were extremely sensitive to external disturbances,and the differential expression was extremely significant.By constructing the metabolic pathway,the decrease/increase of sugar utilization rate during grain filling and the low/overexpression of related protein in nucleotide sugar synthesis pathway reduced/increased the conversion of sugar to starch and the subsequent metabolic process,which may be the key factors limiting the synthesis and accumulation of nutrients in wheat during grain filling.More importantly,the content of α-ketoglutaric acid during grain filling may be the key factor to determine the effect of amino transfer reaction in wheat grain and the biosynthesis of storage protein,which can be used as the substrate for glutenin synthesis.In addition,the content level of storage protein may also depend on the conversion efficiency from pyruvate to acetyl coenzyme A.(4)Results of co-location linkage between coding genes and QTL loci corresponding to differential proteins regulating wheat quality formation are good,and there is a clear close relationship between QTL loci and related regulatory proteins.Through the research results of this topic,it is hoped that the relationship between protein group and metabolomics level among different wheat varieties can be preliminarily revealed,and new ideas can be provided for quality-related gene discovery,molecular marker development,effective utilization of nutrients and artificial regulation and synthesis. |
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