| Conducting genetic diversity research on grapes is of great significance for the development,improvement,breeding,and conservation of grape germplasm resources.In this study,genetic diversity analysis was conducted on 120 superior strains of ’Beihong’ grape initially screened in the field using morphological markers,physiological markers,and SSR molecular markers,in order to provide a basis for selecting new superior strains of ’Beihong’.The results showed that:1.Based on the measurement of 18 morphological indicators for 120 superior "Beihong"grapevines,there are significant differences in various traits among different superior vines.The average coefficient of variation in fruit morphology indicators is 22.35%,and that in leaf morphology indicators is 17.82%.The average coefficient of variation in fruit physiological indicators is 34.89%,with the largest coefficient of variation observed in anthocyanin content(69.40%),followed by total phenols(49.80%)and tannins(42.25%).These coefficients of variation are all above 40%,indicating a high degree of variation in phenolic substances in mature fruits of different superior vines of these 120 ’Beihong’grapevines.2.Cluster analysis showed that superior vines BH-21-100 and BH-21-120 were classified separately in terms of morphology,fruit quality,and molecular clustering and had a large distance from other materials,confirming a close genetic relationship between BH-21-100 and BH-21-120.3.Using SSR molecular markers,11 highly polymorphic and clear-banded primers were selected from 50 primers,and a total of 127 bands were amplified with 11 pairs of primers,of which 79 bands were polymorphic.The amplified fragments were mainly concentrated between 150～750 bp,and the polymorphism rate was 62.45%.The number of alleles N ranged from 2 to 10,with an average of 5.3 alleles per locus;the effective number of alleles Ne ranged from 1.13 to 2.04,with an average of 1.45;Nei’s gene diversity ranged from 0.192 to 0.526,with an average of 0.359;and the Shannon information index I ranged from 0.232 to 0.832,with an average of 0.489,indicating that the tested materials also exhibited rich genetic diversity at the molecular level. |
