Researches On In Vitro Culture Of Camellia Oleifera ’Chang Lin 166’

Camellia oleifera Abel is one of the most important edible oil tree species in China.Camellia oleifera‘Chang Lin 166’,an improved clone variety belonging to Chang Lin series is widely planted in the production region in China.In this paper,researches on in vitro culture of‘Chang Lin 166’were carried out for the purposes of rapid propagation,extension and further genetic improvement of this variety and would be of important theoretic and practical value.Main results were as following:（1）Surface sterilization of explants:for stem segments with axillary buds,it was best to sterilize them with 0.1%HgCl₂ for 7 min,after 20d culture,the contamination rate was 6.67%,the browning rate was 11.00%,and the mortality rate was 14.67%.For leaf explants,it is best to sterilize them with 2.0%NaClO for 25min,after 20d culture,the contamination rate was 8.89%,the browning rate was 20.00%,and the mortality rate was 10.00%.（2）Explants sampling time:the optimum sampling period was from April to June.In this period,the contamination rate,the browning rate and the mortality rate were the lowest,while the germination rate was the highest（75.55%）after 20d culture.（3）Basic medium selection:MS was the best basic medium for stem segments with axillary buds,and the germination rate reached 43.33%after 30d culture.（4）Coagulant and pH of MS media:compared with agar as coagulant and pH was from 5.8 to 6.0,when Phytagel was used as coagulant and pH was from 5.4 to 5.5,better culture was obtained,which germination rate was 44.33%after 30d culture.（5）Leaf browning inhibition:Culture the leaf explants in dark for 5 days could effectively reduce the browning rate to 5.63%after 30d culture.（6）Induction of axillary buds germination:the best medium was MS+6-BA1.5mg/L+NAA 0.5mg/L.After 45 days culture,the germination rate reached 83.34%.（7）Shoots proliferation:the best medium was MS+6-BA 2.0mg/L+NAA 0.1mg/L,the proliferation coefficient was 3.42 after 45 days culture.（8）Callus induction from stem segments:the optimum medium was MS+6-BA2.0mg/L+NAA 0.5mg/L,callus induction rate was 92.33%after 30 days culture.（9）Callus induction from leaves:the optimum medium was MS+6-BA 1.0mg/L+NAA 0.7mg/L,callus induction rate was 95.28%after 30 days culture.（10）Callus induction from petioles:the optimal medium was MS+6-BA 2.5 mg/L+NAA 0.1mg/L,callus induction rate was 71.47%after 30 days culture.（11）Rooting culture and callus induction did not get ideal results,while we had try a variety of test methods.These need to do further study in the latter research.