| Soybean（Glycine max（L.）Merr.）is a phosphorus-loving crop,and phosphorus deficiency is a major factor limiting soybean yield.In agricultural production,phosphorus deficiency is alleviated by applying phosphorus fertilizer,but phosphorus ore is a limited resource and excessive application of phosphorus fertilizer can cause environmental pollution.While growing phosphorus-efficient varieties of soybean is an effective way to solve this problem,it is important to clarify the pathways of phosphorus uptake and translocation in phosphorus-efficient soybean to improve the efficiency of phosphorus utilization in soybean.In this study,based on previous studies,we analyzed the differences in root proteins of soybean with different phosphorus efficiency under low phosphorus stress conditions,and identified the main proteins and metabolic pathways associated with phosphorus deficiency tolerance;screened and validated the reciprocal proteins of the GmPHF1（Glyma.20G190300）gene,and through a preliminary study of its mechanism,thus enriched the regulatory network of processes related to low phosphorus tolerance and the molecular mechanism of root response to low phosphorus stress in soybean.The results of the experimental studies were as follows.1.Expression of genes related to the phosphorus starvation regulatory network in soybean roots with different phosphorus efficiencies was analyzed by sand culture experiments.The expression of phosphorus transporter protein gene GmPHT1 and phosphorus starvation response gene GmPHR1 in phosphorus efficient varieties was significantly lower than that in phosphorus inefficient varieties after a certain treatment time;while the expression of phosphorus transporter protein helper GmPHF1 increased significantly and was significantly higher than that in phosphorus inefficient varieties.In addition,different phosphorus-efficient varieties had different expression patterns of SPX family genes in response to low phosphorus stress.2.Proteomic studies were conducted on soybean roots with different phosphorus efficiency under three phosphorus concentration treatments:no phosphorus(0 mmol·L-1),low phosphorus(0.05 mmol·L-1)and constant phosphorus(0.5 mmol·L-1).A total of 4126proteins were identified in the roots of the two species.The number of differentially expressed proteins obtained from low to no phosphorus conditions increased compared to the normol phosphorus treatment.All differentially expressed proteins obtained in the phosphorus efficient variety were up-regulated in the phosphorus deficient condition,whereas most of the differentially expressed proteins detected in the phosphorus inefficient variety were down-regulated in the phosphorus deficient condition.Meanwhile,three key proteins,I1KW20（forbidden protein）,I1K3U8（α-amylase inhibitor）and C6SZ93（α-amylase inhibitor）,were proposed in this study as potential biomarkers for screening low phosphorus tolerant soybean varieties.3.Under phosphorus stress conditions,phosphorus-inefficient soybean inhibited important metabolic pathways such as root oxidative phosphorylation,glutathione metabolism and carbon metabolism,thereby affecting plant growth.In contrast,phosphorus-efficient varieties increased root metabolic activity,especially in 2-oxoformate metabolism,carbon metabolism,glycolysis,amino acid biosynthesis,pentose phosphorylation,oxidative phosphorylation,other types of O-glycan biosynthesis,and riboflavin metabolic pathways to maintain their normal growth under phosphorus stress conditions.4.Seven proteins interacting with GmPHF1,all of which are involved in plant resistance regulation,were screened using yeast two-hybrid technology,including NAC transcription factors,vesicle-like vesicle-associated proteins,cellulose synthase A catalytic subunit,transcript variants,and anchor protein repeat structural domain proteins.Overall,the results of this study provide new insights into the response and tolerance of soybean to phosphorus deficiency. |
PDF Full Text Request