Research On Analyzing The Morphological Variation Of Ramie Leaves Based On Image Processing Technology

The shape and size of plant leaves are the key to determining the strength of photosynthesis and transpiration,and play an important role in the growth and development of plants.Multi-scale description of blades based on image processing technology has important reference value for multi-directional recognition and understanding of blade morphology.The phenotype study of ramie leaves is helpful for further understanding of ramie germplasm.This paper takes 151 ramie provenances as the research object,and uses image processing technology to observe the phenotypic variation of ramie leaves at the morphological level,in order to provide research references for the genetic diversity of ramie leaves and the breeding of improved varieties.The study obtained the following conclusions:(1)The analysis of the average value of 16 leaf phenotypic traits from 151 ramie provenances found that the leaf phenotypic traits of different provenances are quite different,such as leaf area,leaf length * leaf width The difference between the maximum and minimum average values of,dry matter weight and convex hull area among provenances was 3.33 times,3.41 times,3.37 times and 3.38 times,respectively.Among them,the average value of the three traits of No.V1-16 source leaves,the area of the convex hull,the diameter of the circle,and the length of the minor axis of the normalized ellipse are the largest,and the eccentricity of the normalized ellipse is small(the greater the eccentricity,the flatter the ellipse),it can be inferred that this kind of source has a relatively large leaf shape,which is approximately round;the number V3-22 has the largest elliptical eccentricity,and the leaf area is smaller than the rectangular frame.It can be inferred that this variety has a relatively flat leaf shape and is approximately long egg shape.From the analysis of the coefficient of variation,it can be found that the coefficient of variation of the 13 phenotypic traits of 151 germplasms all showed moderate variation(10<CV<100)or weak variation(1<CV<10).The richness of the coefficient of variation is mainly reflected in leaf length * leaf width,convex hull area,leaf area and ellipse eccentricity.(2)Through the analysis of 16 phenotypic traits of 3020 ramie leaf samples,the study showed that the phenotypic traits of ramie leaves are rich in variation.Except for the leaf area rectangular border ratio traits and the leaf area convex hull ratio traits,the other 14 traits are all It is a moderate degree of variation(10<CV<100);the differences between individuals are extremely significant,and the P values are all <0.01;the distribution of each trait is basically in line with the normal distribution.The phenotypic traits of leaves influence each other,including leaf area,perimeter,leaf width,leaf length,leaf length*leaf width,convex area,circle diameter,dry matter weight traits and 11 other traits except themselves.Shows extremely significant correlations(P<0.01);the correlations between traits are mostly positive correlations,and the three traits of leaf shape index,specific leaf area,and eccentricity have more prominent negative correlations;The shape index traits and the eccentricity traits are extremely significantly positively correlated with each other,and they are negatively correlated with other related traits.Through principal component analysis,leaf phenotypic traits can be divided into three principal components,of which the first principal component is mainly related to the size of the leaf area,indicating that the size of the leaf area is the dominant factor for the leaf phenotypic traits.Followed by the size of the leaf shape and dry weight.(3)Cluster analysis of 151 ramie germplasms on 16 phenotypic traits can be divided into 4 categories.Among them,the first category accounted for the largest proportion,containing 144 germplasm,accounting for 95.36% of the total germplasm.There is only one type in the second and third categories,which are numbered V Zhu Zong No.1 and V Zhong Zhu No.2 respectively.The fourth category contains 5germplasms,namely V1-39,V2-35,V3-12,V1-16 and V3-19,which account for 3.31%of the total germplasm.