Identification And Expression Analysis Of The Melon TCP Transcription Factor Gene Family

Melon is an important cucurbis crop,with important economic value and nutritional value.It is widely distributed,and it has a long history and is one of the top ten fruits in the world.However,At present,my country’s melon industry has generally presented the lag of variety update,lack high quality varieties and a single structure.And the growth and development of melon plants is also affected by factors such as stress and genetic,therefore,strengthening the basic research on the regulation mechanism of melon plant growth and development is of great significance to the future development of melon industry.Studies have shown that the TCP transcription factor family plays a variety of important roles in the entire process of regulating the growth and development of the plant,not only regulates the branches of stems,the development of the flower and leaves and lateral root,the fruit mature,the germination of seeds,etc,but also plays a role in response to hormone signals,biological and non-biological stress.In this study,Biological information analysis by the melon TCP transcription factor family,in order to understand its protein characteristics,chromosome positioning,protein secondary structure,and tertiary structures,subcellular positioning,genetic structure and conservative motifs,evolutionary trees,cis-cating elements,GO enrichment,protein interaction network analysis,mi RNA prediction and gene expression patterns,etc.It provides a theoretical basis for studying the function of TCP family genes in the growth and development of melon,lays a theoretical foundation for further in-depth research on the branching development of melon plants in the later stage.The main results of this study are as follows:1.In the whole genome of melon,29 TCP family members containing typical TCP domains were screened and identified,distributed unequally on 9 chromosomes of melon,with amino acid sequence length ranging from 61 to 600 aa,and named according to the physical location of genes on chromosomes and the naming method of TCP genes in Arabidopsis.Ex PASy was used to analyze the physical and chemical properties of the TCP gene family members of melon.The results showed that the molecular weight and isoelectric point of 29 Cm TCPs proteins were significantly different,and 86.21% of Cm TCPs proteins PI > 7,indicating that most of them were basic proteins.93.31% of Cm TCPs proteins were unstable,and all Cm TCPs proteins were hydrophilic.The prediction results of the protein’s transmembrane structure and signal peptide showed that only Cm TCP17 has a transmembrane structure and signal peptide among the members of the Cm TCPs protein family in melon.The prediction analysis of phosphorylation sites indicated that there were some differences in the number of phosphorylation sites among Cm TCPs protein family members,except for Cm TCP4,the number of phosphorylation sites of the remaining members were all serine>threonine>tyrosine.The results of subcellular localization prediction analysis showed that Cm TCP protein was mainly located in the nucleus and only one protein was located in the cytoplasm.The predicted results of the secondary structure and tertiary structure of the protein were in good agreement.The analysis results showed that the main structural element of the melon TCP transcription factor family protein was random coil.2.In order to determine the phylogenetic relationship of the members of the melon TCP family gene,a phylogenetic tree was constructed by multiple sequence alignment with the TCP family genes of watermelon,cucumber and Arabidopsis thaliana.According to the analysis of clustering results,the melon TCP gene family was divided into two subfamilies,Class I subfamily and Class II subfamily,and the Class II subfamily was further divided into CYC and CIN subgroups according to the conserved domains.3.The analysis of gene structure showed that the TCPs gene structure of melon was relatively simple,containing 1-2 exons.There was no significant difference in the gene structure of Cm TCPs gene in the same subgroup,but there were differences among different subgroups.Conservative motif analysis showed that all 29 Cm TCP genes contained motif1,but different subgroups contained different kinds of motifs and motif combinations,which could be used as markers to distinguish different subgroups.4.The results of cis-acting element analysis showed that the TCP gene family of melon mainly contained light-responsive elements,growth-regulation-related elements,and hormone-and stress-related response elements.GO enrichment analysis showed that the Cm TCP gene family was mainly involved in the regulation of organ development,branch development,leaf development,bud system formation and other growth and development processes,as well as the regulation of response to hormones and external stimuli.5.The results of protein interaction network analysis showed that the interacting proteins of the same subgroup of Cm TCP proteins were the same,and not only interacted with proteins in the same family,but also interacted with the same subgroup of proteins,and also interacted with other proteins.The mi RNA prediction analysis of Cm TCPs gene showed that 13 target genes corresponding to 26 mi RNAs were obtained.It was speculated that mi RNAs may play an important role in the physiological and biochemical metabolic processes regulated by the melon TCP gene.6.Transcriptome data analysis of Cm TCP gene expression in melon showed that all 27 Cm TCP genes could be expressed in 5 tissues of melon,and there were significant differences in the expression of genes in tissues.The same subgroup of highly similar patterns of gene expression in the same organization,different subgroups with differences in gene expression in the tissues,showed that the same group of genes have similar gene functions.It implied that the melon TCP family genes are involved in the growth and development of melon and the different group members have different division of labor regulation genes.In this study,q RT-PCR analysis showed that members of cm TCP-CYC /TB1 subgroup were differentially expressed in 6 tissues of 2 materials,and all genes could be detected in stem tissues.The results of this study also provide important clues and basis for further research on the function of the TCP gene in melon.