| Camelina sativa is an important economic oil crop with the characteristics of high seed oil content,short plant growth period,disease resistance and barren resistance.NAC(NAM 、ATAF1/2 、 CUC1/2)transcription factors play an important role in plant adaptation to the environment and resistance to adversity conditions.333 NAC proteins were identified in Camelina sativa by HMMER search and conserved domain analysis.Referring to the existing NAC subfamily information in Arabidopsis thaliana,these proteins were divided into 20 subfamilies in the phylogenetic tree.These proteins have characteristic domains of NAC transcription factors,but there are differences in the number of introns in these genes.The results of evolutionary analysis revealed that segmental duplication was the main driving force for the family expansion.These proteins are highly hydrophilic,and most of them are unstable acidic proteins.Only the protein of Cs NAC146 was predicted to have a signal peptide sequence,and 36 proteins had a membrane-binding domain,most of which were at the C-terminus.The subcellular localization was mainly located in the nucleus.The analysis of cis-acting elements showed that the Camelina sativa NAC family play an important role in abiotic stress,growth and development and regulation of hormone signaling pathways.The structures of proteins are dominated by random coils,the three-dimensional structure of the same subfamily proteins is highly similar,and the structure is relatively conservative.The GO function prediction results confirmed that the NAC transcription factors have DNA-binding region.The codon bias is not strong and there is co-expression in the Camelina sativa NAC family.The results of q PCR expression analysis showed that the genes with higher expression levels could respond to salt stress and drought stress.The gene expression levels generally showed an increasing trend under salt stress conditions.The evolution analysis,physicochemical properties and structure analysis and expression level analysis of the Camelina sativa NAC family were carried out,which provided a theoretical basis for further in-depth study of the functions and mechanisms,and provided a new reference for the systematic study of the Camelina sativa NAC family.Figure 26;Table 7;Reference 81... |
