Effects Of Additives And Peanut Vine On Silage Quality Of "Tifton 85" Bermudagrass

This experiment was conducted to study the effects of different concentrations of lactic acid bacteria,cellulase and their combination on the nutritional quality,fermentation quality,microbial number and aerobic stability of“Tifton 85”bermudagrass silage.Meanwhile,“Tifton 85”bermudagrass with high water soluble carbohydrate content and high crude protein content of peanut vine were mixed silage at different proportions to explore the optimal mixing ratio,which provided the basis for establishing the high quality silage technology of“Tifton 85”bermudagrass and peanut vine.fourteen treatments,including control,lactic acid bacteria(0.05 g·kg^-1,0.10 g·kg^-1,0.15 g·kg^-1),cellulase(0.05 g·kg^-1,0.10 g·kg^-1,0.15 g·kg^-1),lactic acid bacteria+cellulase(0.05 g·kg^-1+0.05 g·kg^-1,0.10 g·kg^-1+0.10 g·kg^-1,0.15 g·kg^-1+0.15 g·kg^-1),and“Tifton 85”bermudagrass:peanut vine（75:25、50:50、25:75、0:100）,were designated,and set three replicates for each treatment.After sealed storage for 60 days,the nutrient quality:dry matter,crude protein,crude fat,soluble carbohydrate,acid detergent fiber and neutral detergent fiber;Fermentation quality:pH value,ammonia nitrogen/total nitrogen,lactic acid,acetic acid,propionic acid,butyric acid;Number of microorganisms:lactic acid bacteria,yeast,mold;Aerobic stability,pH value,ammonia nitrogen/total nitrogen and microbial number at 3,6,9,12 and 15 days of aerobic exposure of silage was determined.The results show that:1)The contents of acid detergent fiber and neutral detergent fiber of“Tifton 85” bermudagrass silage with lactic acid bacteria were significantly higher than those of the control（P<0.05）.With the increase of lactic acid bacteria concentration,sensory evaluation score,the contents of acid detergent fiber and neutral detergent fiber increased.The crude protein content of the treatment with 0.15 g·kg^-1lactic acid bacteria was significantly higher than that of the control（P<0.05）.The pH value of lactic acid bacteria treatment were decreased and it’s lactic acid content were increased,but there was no significant difference between 0.05 g·kg^-1lactic acid bacteria treatment and control（P>0.05）.Ammonia nitrogen/total nitrogen was significantly decreased with the increase of lactic acid bacteria concentration（P<0.05）.Adding lactic acid bacteria could significantly increase the number of lactic acid bacteria and aerobic stability（P<0.05）.2)Adding cellulase could significantly increase crude protein content and decrease acid detergent fiber and ammonia nitrogen content of silage（P<0.05）.The pH value of 0.05g·kg^-1cellulase was significantly lower than that of the control,and the content of lactic acid,the number of lactic acid bacteria and aerobic stability were significantly higher than those of the control（P<0.05）.The pH value,lactic acid content,and number of lactic acid bacteria of 0.10 g·kg^-1and 0.15 g·kg^-1cellulase were not significantly different from the control（P>0.05）.3)The addition of lactic acid bacteria and cellulase combination significantly increased the silage crude protein content and significantly reduced the neutral detergent fiber content（P<0.05）.The pH values of 0.05 g·kg^-1lactic acid bacteria+0.05 g·kg^-1cellulase and 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase were significantly lower than that of other treatment groups（P<0.05）,but there was no significant difference between them,and the pH value of 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase treatment were the lowest.Except for 0.05 g·kg^-1cellulase,ammonia nitrogen/total nitrogen,of 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase was significantly lower than that of other treatment groups（P<0.05）.The content of lactic acid,acetic acid of 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase was the highest,which was significantly different than that in other groups（P<0.05）,and no butyric acid was produced.The number of lactic acid bacteria of the treatment with 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase was significantly higher than that in the other groups（P<0.05）.Except 0.15 g·kg^-1lactic acid bacteria,the aerobic stability of 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase was significantly higher than that of others（P<0.05）.4)The sensory score,crude protein content,lactic acid content and the number of lactic acid bacteria of“Tifton 85”bermudagrass mixed with peanut vine at a ratio of 25:75 were significantly higher than those of other treatment groups,while pH value,ammonia nitrogen/total nitrogen and the number of yeast were significantly lower than those of other treatments（P<0.05）.Mixed treatments significantly increased the aerobic stability of silage（P<0.05）,and the highest aerobic stability was obtained when the ratio of bermudagrass and peanut vine was 25:75.In conclusion,lactic acid bacteria,cellulase and their combination can improve the nutrient quality,fermentation quality,aerobic stability,and increase the number of lactic acid bacteria of silage.The treatment with 0.10 g·kg^-1lactic acid bacteria+0.10 g·kg^-1cellulase has the best effect.The ratio of bermudagrasss and peanut vine is 25:75 has the highest nutrient quality,fermentation quality and aerobic stability,and it’s the number of lactic acid bacteria is the highest,so it’s has the best effect among mixed silage.