Effects Of Different Breeding Models On Growth Performance,Meat Quality,and Related Metabolism Of Ningxia Tan Sheep

To explore the effects of the growth and slaughter,meat quality,rumen internal environment,microorganism,blood biochemistry and antioxidant index of Tan sheep by different breeding methods,with a view to provide a theoretical basis for the selection of mode of production,improvement of production performance and improvement of mutton quality in Tan sheep production practice.The research is intended for the study of the Tan sheep,selecting 24 emasculated male Tan sheep of healthy and similar weight divided into 3 groups with 8 sheep in each group.Drylot breeding group(C group)was fed with total mixed diet,and grazing supplementary breeding group(CG group)was grazed after supplying beneficiated burden material,the grazing group(G group)grazed grassland.The pre-breeding period is 15 days,the formal period is 90 days.The research results of production,slaughter performance and the quality of meat of Tan sheep indicate that:(1)Compared with G group,C group and CG group,significantly increased Tan sheep’s end weight,daily gain,carcass weight,dressing percentage and back mark thickness(P<0.05).(2)C group than CG and G group significantly increased the fat content in meat,it was 3.73%(P<0.05).The protein content of CG group was higher than that of C group,it was 22.66%(P<0.05).C group and CG group than G group significantly increased 24h pH(P<0.05).The meat quality score of CG group was higher than that of C group and G group.(3)There was no significant difference in net profit between groups C and CG,increasing by 1.81 yuan/(a*d)and 1.61 yuan/(a*d)compared with group G,respectively.The results of ruminal environment,microorganism and morphology of gastrointestinal tract of Tan sheep showed that:(1)The content of acetic acid in C group was significantly lower than that in CG group and G group(P<0.05).The isobutyric acid content in CG group was significantly higher than that in C group(P<0.05).(2)The bacteroidetes content of G group was 61.58%,were significantly higher than those in C and CG group(P<0.05),there was a significant negative correlation with EE,daily gain and dressing rate(P<0.05),there are also significant positive correlation with 24h pH(P<0.05).The content of proteobacteria in Group C was 28.12%,were significantly higher than CG and G(P<0.05),It was positively correlated with EE,daily gain and dressing rate(P<0.05).The content of fibrinogen and actinomycetes in CG group was significantly higher than that in C group and G group(P<0.05).The contents of Succinivibrio and Succinivibrionaceae_UCG002 in C group were 18.76%and 7.30%,which were significantly higher than those in CG group and G group(P<0.05).The contents of Prevotellaceae_UCG001 and Succinivibrionaceae_UCG001 in CG group were significantly higher than those in C group and G group(P<0.05).(3)The height of Duodenum Villi in C group was significantly higher than that in C group and G group(P<0.05).The thickness of duodenum muscle layer in G group was significantly higher than that in C group and G group(P<0.05).There was no significant difference in the morphology and structure of rumen in each group(P>0.05).The results of blood biochemical index and anti-oxidation index of Tan sheep showed that:(1)Serum total cholesterol(TC)in CG group was significantly lower than that in C group(P<0.05),TC was 1.02 mmol/L.Urea nitrogen(UREA)content in CG group and G group was significantly higher than that in C group(P<0.05).Serum creatinine levels in CG group were significantly higher than those in C group and G group(P<0.05).Urea was negatively correlated with slaughter rate(P<0.05),blood Creatinine was positively correlated with CP(P<0.05),(2)The content of MDA(MDA)in CG group was significantly higher than that in C group(P<0.05).The content of Catalase(CAT)and Superoxide dismutase(SOD)in CG group was significantly higher than that in C group and G group.The total antioxidant capacity(T-AOC)of CG group and G group was significantly higher than that of C group(P<0.05).The glutathione peroxidase(GSH-PX)in G group was significantly lower than that in CG group(P<0.05).The results of extensive targeted metabonomics analysis of tan-sheep’s blood show that:760 metabolites were detected in tan sheep plasma,compared with G group,8 different metabolites were screened out in C group,and 34 different metabolites were screened out in C group and G group Compared with CG group,15 different metabolites were screened in G group.In the participating immune,anti-inflammatory,antioxidant-related metabolites,It shows that grazing supplementary breeding and grazing breeding style enhance the increase of immune,anti-inflammatory and antioxidant plasma-related metabolites of beach sheep compared with drylot breeding method;In the participating lipid-related metabolites,This indicates that the increase of plasma associated metabolites of hepatocyte injury compared with other practices,drylot breeding and grazing supplementary breeding are beneficial to increase cholesterol and plasma related metabolite content compared with grazing;Related metabolites involved in growth-promoting metabolism,It shows that drylot breeding and grazing supplementary breeding methods promote the increase of plasma-related metabolites content of beach sheep feed intake compared with grazing,Compared with grazing breeding and grazing supplementary breeding breeding can promote cell proliferation and immune response.In conclusion,compared with grazing breeding methods,grazing and supplementary breeding methods and house breeding methods can also improve the production and slaughter performance.The grazing and supplementary breeding method can improve the meat quality,rumen environment and the antioxidant ability of Tan sheep to the certain extent.In addition,different breeding methods can also have certain effects on plasma metabolism.Therefore,the selection of grazing and supplementary breeding in the production practice is more conducive to the development of Ningxia Tan sheep.