Effect Of FGF2 On The Antioxidant And Sugar Metabolism Capacity Of Oocytes In Mice Under Oxidative Stress

Basic fibroblast growth factor（fibroblast growth factor 2）is a member of fibroblast growth factor superfamily.Studies have shown that FGF2 can not only repair nerve cells and tissue cells,but also resist oxidative stress in cardiomyocytes and brain cells.The purpose of this study is to explore whether FGF2 can improve the function of mitochondria and improve the aerobic glucose metabolism pathway through PI3K-AKT1-HIF signal pathway,so as to protect oxidative stress oocytes and improve the quality of oocytes.The rats were divided into three groups:control group（0 ng/ml FGF2+0μmol/L H₂O₂）,H₂O₂ group（250μmol/L H₂O₂+0ng/ml FGF2）and antioxidant group（150 ng/ml FGF2+250μmol/L H₂O₂）.After the collected oocytes were added to the three groups of culture medium for 24 hours,the markers of oxidative stress（ROS,MDA,8-OHd G）and reduced glutathione（GSH）were detected to verify the effect of FGF2 on oxidative stress of oocytes,and then the mitochondrial function of oocytes was detected（mitochondrial membrane potential,ATP,ADP,AMP,energy charge）.Finaly,the activities of PI3K-AKT-HIF and key enzymes in glucose metabolism pathway were detected by Westernblot and enzyme-linked immunosorbent assay（Elisa）.The final results are as follows:1.The results of the comparison of the contents of oxidative stress markers in the oocytes showed that the content of 8-OHd G, which was significantly different among the three groups（P<0.05）;ROS content,the H₂O₂ group was significantly higher than the antioxidant and control groups（P<0.05）,and the difference between the antioxidant and control groups was not significant（P>0.05）;MDA content,which was significantly different among the three groups（P<0.05）.2.Intracellular GSH levels in oocytes:significant differences were observed among the three groups（P<0.05）.3.Intracelular mitochondrial membrane potential and energy load level:the differences among the three groups were significant（P<0.05）.4.The level of pi3kca phosphorylation,which was significantly different among the three groups（P<0.05）;In addition,Akt expression,which was significantly lower in the H₂O₂ group than in the other two groups（P<0.05）,did not differ between the antioxidant and control groups（P>0.05）.5.Effects of FGF2 on enzymatic activities of glucose metabolism in oocytes:enzyme activities of HK,6-PFK,and PK were significantly decreased in the control versus antioxidant groups,whereas the opposite was true for PDK1（P<0.05）.In the antioxidant group,the enzymatic activities of 6-PFK and PDK1 were all restored to normal levels（P>0.05）,while the enzymatic activities of HK and PK were still significantly lower than those of the control group（P<0.05）.6.The content of HIF in the H₂O₂ group was significantly higher than that in the control group（P<0.05）,in the antioxidant group was significantly lower than that in the H₂O₂ group（P<0.05）,and there was no significant difference between the control group and the antioxidant group（P>0.05）.Conclusion:FGF2 can protect mouse oocytes from oxidative stress by increasing the level of GSH,reducing the production of oxidative stress and improving mitochondrial function,and through repairing PI3K/AKT-HIF pathway,reduce the activity of anaerobic glycolysis pathway（EMP）enzyme and promote the activity of glucose aerobic metabolism pathway（TCA）enzyme,thus improve the quality of mouse oocytes.